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niman
 Post subject: Re: trH3N2 Case In Maine
PostPosted: Mon Oct 17, 2011 7:52 pm 
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Not many pigs in Maine.

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niman
 Post subject: Re: trH3N2 Case In Maine
PostPosted: Mon Oct 17, 2011 7:53 pm 
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Location: Pittsburgh, PA USA
Expect flood gates to open soon on human trH3N2. CDC and WHO will soon be forced to acknowledge pandemic.

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niman
 Post subject: Re: trH3N2 Case In Maine
PostPosted: Mon Oct 17, 2011 7:55 pm 
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Location: Pittsburgh, PA USA
Pandemic H1N1 MP on different human trH3N2 backgrounds signals major problems.

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niman
 Post subject: Re: trH3N2 Case In Maine
PostPosted: Mon Oct 17, 2011 8:07 pm 
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Location: Pittsburgh, PA USA
Maine CDC

Influenza, novel
Influenza, novel
Positive influenza A, unable to serotype
or novel strain
Y
es
PCR screening A/B and PCR subtyping H1N1, H3N2, H5, culture, Drug Resistance by Pyrosequencing (2009 H1N1) (Available in 2010-11 season)

http://www.maine.gov/dhhs/boh/ddc/epi/d ... 2011_2.pdf

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niman
 Post subject: Re: trH3N2 Case In Maine
PostPosted: Mon Oct 17, 2011 8:22 pm 
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Location: Pittsburgh, PA USA
PB1 does not have E618D. Constellation matches 2011 cases but not as tighly clustered as Indiana and Pennsylvania sequences.

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niman
 Post subject: Re: trH3N2 Case In Maine
PostPosted: Mon Oct 17, 2011 8:47 pm 
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Location: Pittsburgh, PA USA
Commentary

http://www.recombinomics.com/News/10181 ... Maine.html

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niman
 Post subject: Re: trH3N2 Case In Maine
PostPosted: Mon Oct 17, 2011 9:41 pm 
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Location: Pittsburgh, PA USA
Multiple HA polymorphisms in 29 BP segment

EPI339171 A/Maine/06/2011 (A/H3N2) segment 4 (HA) 54.7 7.571566e-08 29/29 (100%)
EPI338562 A/swine/Indiana/A01049744/2011 (A/H3N2) segment 4 (HA) 54.7 7.571566e-08 29/29 (100%)
EPI338561 A/swine/Indiana/A01049745/2011 (A/H3N2) segment 4 (HA) 54.7 7.571566e-08 29/29 (100%)
EPI338421 A/swine/Indiana/A01049653/2011 (A/H3N2) segment 4 (HA) 54.7 7.571566e-08 29/29 (100%)
EPI335629 A/Indiana/08/2011 (A/H3N2) segment 4 (HA) 54.7 7.571566e-08 29/29 (100%)
EPI335618 A/Pennsylvania/11/2011 (A/H3N2) segment 4 (HA) 54.7 7.571566e-08 29/29 (100%)
EPI335610 A/Pennsylvania/09/2011 (A/H3N2) segment 4 (HA) 54.7 7.571566e-08 29/29 (100%)
EPI333152 A/Indiana/08/2011 (A/H3N2) segment 4 (HA) 54.7 7.571566e-08 29/29 (100%)
EPI324857 A/swine/North Carolina/A01049436/2011 (A/H3N2) segment 4 (HA) 54.7 7.571566e-08 29/29 (100%)
EPI317003 A/swine/Indiana/A0109091/2010 (A/H3N2) segment 4 (HA) 54.7 7.571566e-08 29/29 (100%)

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niman
 Post subject: Re: trH3N2 Case In Maine
PostPosted: Mon Oct 17, 2011 11:27 pm 
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Location: Pittsburgh, PA USA
Maine case has minor variations, but it is essentially the same as Indiana and Pennsylvania sequences, once again signaling H2H transmission,

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niman
 Post subject: Re: trH3N2 Case In Maine
PostPosted: Tue Oct 18, 2011 12:55 am 
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Location: Pittsburgh, PA USA
Commentary

http://www.recombinomics.com/News/10181 ... IN_PA.html

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niman
 Post subject: Re: trH3N2 Case In Maine
PostPosted: Tue Oct 18, 2011 6:43 am 
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Location: Pittsburgh, PA USA
Tandem PB1 polymorphisms T1356C and A1377C

EPI339169 A/Maine/06/2011 (A/H3N2) segment 2 (PB1) 41.7 2.531566e-04 22/22 (100%)
EPI335627 A/Indiana/08/2011 (A/H3N2) segment 2 (PB1) 41.7 2.531566e-04 22/22 (100%)
EPI335616 A/Pennsylvania/11/2011 (A/H3N2) segment 2 (PB1) 41.7 2.531566e-04 22/22 (100%)
EPI335608 A/Pennsylvania/09/2011 (A/H3N2) segment 2 (PB1) 41.7 2.531566e-04 22/22 (100%)
EPI333150 A/Indiana/08/2011 (A/H3N2) segment 2 (PB1) 41.7 2.531566e-04 22/22 (100%)

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