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niman
PostPosted: Wed Apr 27, 2011 8:54 am 
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niman wrote:
gsgs wrote:
> The Air Force has already demonstrated that the Chihuahua sub-clade
> has evolved AWAY from existing immunity to pandemic H1N1
> (and a vaccine targeting California/07). January was the herald wave.

what exacly did "The Air Force" say ? Do you have a link ?

(and what do they know)

((do CDC,WHO,ECDC,PAHO confirm ??))

FDA transcript of 2011 vaccine meeting

http://www.fda.gov/advisorycommittees/c ... 249303.htm

DR. GELLIN: I am always impressed by what a body of work that is. Two questions. One of them, you had mentioned about the low reactors and the biological issues at the lab in London, is that the same issue for the H1 as well as the H3? Because they showed, I think, that there was a higher percentage of H1 low reactors in the London lab, as well. The question really is that a lab problem or does that signal some other evolution, since they have had more H1 this year?
DR. COX: Yes. What we know for H1N1 viruses is that if you isolate them in a particular cell line, you tend to get more of the variance with changes within that region that I mentioned -- the 153 to 158 region. They had been using the particular cell line that pulls out that particular variant or that selects for that particular variant.
They have subsequently switched to normal MDCK cells. I think what they are seeing is a combination of the two phenomena. Plus, they receive a lot of viruses that have been isolated COT, the special kind of MDCK cells. I think it is a combination of they are receiving viruses with changes that cause that reduction in activity and then having some additional low reactors, but they are scattered throughout the tree.
Note that when assays are used that are more sensitive (by Mill Hill), the CDC pushes to use cell lines that are LESS sensitive and therefore produce more MATCHES for sequences that are UNMATCHED (low reactors).

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niman
PostPosted: Wed Apr 27, 2011 8:58 am 
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DR. COX: Good question. I think I can answer it very clearly. We have developed a technique that can screen for reassortants and we are not seeing reassortment between the old H1N1 and the new H1N1 or between the new H1N1 and H3N2 human viruses.

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niman
PostPosted: Wed Apr 27, 2011 9:10 am 
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Location: Pittsburgh, PA USA
REALITY:

The military recruits, again, a captive population, essentially 100 percent immunized and, for the purposes of this slide, 100 percent immunized with the LAIV. Two sites that did receive TIV were excluded from this analysis.
We had a very remarkable increase in infections with pandemic H1N1 in recent weeks in our recruits. That is very unusual for us. Highly vaccinated, we do see influenza in that highly vaccinated population. We take those seriously, in case they are breakthroughs to see if there is drift of the circulating strains from the vaccine. But this is very unusual to see so many of late.

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niman
PostPosted: Wed Apr 27, 2011 9:15 am 
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Location: Pittsburgh, PA USA
niman wrote:
REALITY:

The military recruits, again, a captive population, essentially 100 percent immunized and, for the purposes of this slide, 100 percent immunized with the LAIV. Two sites that did receive TIV were excluded from this analysis.
We had a very remarkable increase in infections with pandemic H1N1 in recent weeks in our recruits. That is very unusual for us. Highly vaccinated, we do see influenza in that highly vaccinated population. We take those seriously, in case they are breakthroughs to see if there is drift of the circulating strains from the vaccine. But this is very unusual to see so many of late.

Note that the AIR FORCE STILL has not released 2011 H1N1 sequences from Texas.

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gsgs
PostPosted: Wed Apr 27, 2011 9:21 am 
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Location: germany
all H1 viruses at genbank marked as "previously vaccinated"
seem to be from this season and "AF" for airforce

Code:
                                            0000000000000000000000000000000000000000000000000000111111111
                                            0000000011111233333444455555566666666666778888899999000111111
                                            0124667934488502349005713356900022456788254568802455568234779
                                            6625042652459534606891861653856707083814669554531567680216124
-codon-position----------------------------- 11  1   1 2     1  111  2 212 12 11        12    2       12 
---Index------------------------------------GGCAAATTCACGGATCCGGGAGAAGGTATGTGGTGTAGCGGGCAGCTTCCAATGGGAGGAT
   1 >A/Mexflu/index/2009/02/01             .............................................................
   4 >A/Korea/AF21780/2010/12/24            ......C...T.........G.G....G.C..T.AA................C.....A..
   7 >A/Korea/AF21783/2010/12/29            ......C...T..T........G...C..C....AA...A............C..A..A..
   9 >A/Japane/AF21777/2011/01/13           ......C...T...........G......C...CAA................C.....A..
  10 >A/Korea/AF21784/2011/01/04            A.........T...........G......C....AA.............A..C.....A.A
  11 >A/Korea/AF21785/2011/01/10            ......C...T.....T..A..G......C....AA................C.....A..
  13 >A/Korea/AF21787/2011/01/04            ..........T.A.........G......C....AA................C....TA..
  14 >A/Korea/AF21788/2011/01/05            A...G.....T...........G......C....AA.............A..C.....A.A
  15 >A/Korea/AF21789/2011/01/07            A...G.....T...........G......C....AA.............A..C.....A.A
  16 >A/Korea/AF21790/2011/01/09            ..A.......T........A..G.....CC....AA................C.....A..
                                                                                                         
  12 >A/Korea/AF21786/2011/01/04            ...C...CT........A.T.........CC....A.....T.G........C.....A..
   2 >A/Korea/AF21778/2010/12/23            ...........A.................C.....A.A..............CA....A..
                                                                                                         
   3 >A/Korea/AF21779/2010/12/24            .....................A..A...C......A............T.......G....
   5 >A/Korea/AF21781/2010/12/21            .....................A..A...C......A............T.......G....
   6 >A/Korea/AF21782/2010/12/29            .....................A..A...C......A.........A..T.......G....
  18 >A/North Carolina/AF21797/2011/01/20   .....................A......C......A.A............G........G.
                                                                                                         
   8 >A/Arizona/AF21768/2011/01/07          .A...G...G.....T..A....G.A.....A...A..T.A.T.A..G...G..A......
  17 >A/New Jersey/AF21791/2011/01/13       .........G....C...A......A.....A...AT.T.....A.CG...G..A......
                                                                                                         
---Index------------------------------------GGCAAATTCACGGATCCGGGAGAAGGTATGTGGTGTAGCGGGCAGCTTCCAATGGGAGGAT
-codon-position----------------------------- 11  1   1 2     1  111  2 212 12 11        12    2       12 
                                            0000000000000000000000000000000000000000000000000000111111111
                                            0000000011111233333444455555566666666666778888899999000111111
                                            0124667934488502349005713356900022456788254568802455568234779
                                            6625042652459534606891861653856707083814669554531567680216124
                                                                                                         
.
.

.

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niman
PostPosted: Wed Apr 27, 2011 9:39 am 
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Joined: Wed Aug 19, 2009 10:42 am
Posts: 27370
Location: Pittsburgh, PA USA
gsgs wrote:
all H1 viruses at genbank marked as "previously vaccinated"
seem to be from this season and "AF" for airforce

Code:
                                            0000000000000000000000000000000000000000000000000000111111111
                                            0000000011111233333444455555566666666666778888899999000111111
                                            0124667934488502349005713356900022456788254568802455568234779
                                            6625042652459534606891861653856707083814669554531567680216124
-codon-position----------------------------- 11  1   1 2     1  111  2 212 12 11        12    2       12 
---Index------------------------------------GGCAAATTCACGGATCCGGGAGAAGGTATGTGGTGTAGCGGGCAGCTTCCAATGGGAGGAT
   1 >A/Mexflu/index/2009/02/01             .............................................................
   4 >A/Korea/AF21780/2010/12/24            ......C...T.........G.G....G.C..T.AA................C.....A..
   7 >A/Korea/AF21783/2010/12/29            ......C...T..T........G...C..C....AA...A............C..A..A..
   9 >A/Japane/AF21777/2011/01/13           ......C...T...........G......C...CAA................C.....A..
  10 >A/Korea/AF21784/2011/01/04            A.........T...........G......C....AA.............A..C.....A.A
  11 >A/Korea/AF21785/2011/01/10            ......C...T.....T..A..G......C....AA................C.....A..
  13 >A/Korea/AF21787/2011/01/04            ..........T.A.........G......C....AA................C....TA..
  14 >A/Korea/AF21788/2011/01/05            A...G.....T...........G......C....AA.............A..C.....A.A
  15 >A/Korea/AF21789/2011/01/07            A...G.....T...........G......C....AA.............A..C.....A.A
  16 >A/Korea/AF21790/2011/01/09            ..A.......T........A..G.....CC....AA................C.....A..
                                                                                                         
  12 >A/Korea/AF21786/2011/01/04            ...C...CT........A.T.........CC....A.....T.G........C.....A..
   2 >A/Korea/AF21778/2010/12/23            ...........A.................C.....A.A..............CA....A..
                                                                                                         
   3 >A/Korea/AF21779/2010/12/24            .....................A..A...C......A............T.......G....
   5 >A/Korea/AF21781/2010/12/21            .....................A..A...C......A............T.......G....
   6 >A/Korea/AF21782/2010/12/29            .....................A..A...C......A.........A..T.......G....
  18 >A/North Carolina/AF21797/2011/01/20   .....................A......C......A.A............G........G.
                                                                                                         
   8 >A/Arizona/AF21768/2011/01/07          .A...G...G.....T..A....G.A.....A...A..T.A.T.A..G...G..A......
  17 >A/New Jersey/AF21791/2011/01/13       .........G....C...A......A.....A...AT.T.....A.CG...G..A......
                                                                                                         
---Index------------------------------------GGCAAATTCACGGATCCGGGAGAAGGTATGTGGTGTAGCGGGCAGCTTCCAATGGGAGGAT
-codon-position----------------------------- 11  1   1 2     1  111  2 212 12 11        12    2       12 
                                            0000000000000000000000000000000000000000000000000000111111111
                                            0000000011111233333444455555566666666666778888899999000111111
                                            0124667934488502349005713356900022456788254568802455568234779
                                            6625042652459534606891861653856707083814669554531567680216124
                                                                                                         
.
.

.

The US isolates above are the Chihuahua sub-clade.

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niman
PostPosted: Wed Apr 27, 2011 9:42 am 
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Joined: Wed Aug 19, 2009 10:42 am
Posts: 27370
Location: Pittsburgh, PA USA
niman wrote:
REALITY:

The military recruits, again, a captive population, essentially 100 percent immunized and, for the purposes of this slide, 100 percent immunized with the LAIV. Two sites that did receive TIV were excluded from this analysis.
We had a very remarkable increase in infections with pandemic H1N1 in recent weeks in our recruits. That is very unusual for us. Highly vaccinated, we do see influenza in that highly vaccinated population. We take those seriously, in case they are breakthroughs to see if there is drift of the circulating strains from the vaccine. But this is very unusual to see so many of late.

The above is reality representing massive H1n1 breakthrough. Below are the latest fake numbers by the CDC (from FluView) on H1N1 low reactors (1 out of 481 tested)
2009 Influenza A (H1N1) [481]

•Four hundred eighty (99.8%) of the 481 tested were characterized as A/California/7/2009-like, the influenza A (H1N1) component of the 2010-11 influenza vaccine for the Northern Hemisphere. One virus (0.2%) of the 481 tested showed reduced titers with antiserum produced against A/California/7/2009.

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Dingo
PostPosted: Wed Apr 27, 2011 9:44 am 
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Posts: 887
niman wrote:
REALITY:

The military recruits, again, a captive population, essentially 100 percent immunized and, for the purposes of this slide, 100 percent immunized with the LAIV. Two sites that did receive TIV were excluded from this analysis.
We had a very remarkable increase in infections with pandemic H1N1 in recent weeks in our recruits. That is very unusual for us. Highly vaccinated, we do see influenza in that highly vaccinated population. We take those seriously, in case they are breakthroughs to see if there is drift of the circulating strains from the vaccine. But this is very unusual to see so many of late.


Where's that from?
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gsgs
PostPosted: Wed Apr 27, 2011 9:46 am 
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Location: germany
unfortunately that document is very long and no summary

seems that the military had bad effectiveness of the H1-part
of LAIV

live attenuate influenza vaccine
("flumist"? , usually given to children in USA)

TIV = trivalent influenza vaccine
(normal vax)

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Last edited by gsgs on Wed Apr 27, 2011 9:50 am, edited 1 time in total.

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niman
PostPosted: Wed Apr 27, 2011 9:48 am 
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Joined: Wed Aug 19, 2009 10:42 am
Posts: 27370
Location: Pittsburgh, PA USA
Dingo wrote:
niman wrote:
REALITY:

The military recruits, again, a captive population, essentially 100 percent immunized and, for the purposes of this slide, 100 percent immunized with the LAIV. Two sites that did receive TIV were excluded from this analysis.
We had a very remarkable increase in infections with pandemic H1N1 in recent weeks in our recruits. That is very unusual for us. Highly vaccinated, we do see influenza in that highly vaccinated population. We take those seriously, in case they are breakthroughs to see if there is drift of the circulating strains from the vaccine. But this is very unusual to see so many of late.


Where's that from?

This is from the Air Force presentation at the US vaccine selection meeting, where there was one abstension and the rest voted to AGAIN use California/07/2009 as the H1N1 target for 2011/2012.
Full transcript is here
http://www.fda.gov/advisorycommittees/c ... 249303.htm

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