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 Post subject: Fit H274Y in Tennessee
PostPosted: Tue Dec 01, 2009 10:51 am 
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Posts: 27362
Location: Pittsburgh, PA USA
CDC just deposited a series of new sequences at GISAID. Several have Tamiflu resistance. On, A/Tennessee/17/2009, is the same two markers as the evolunionarily fit Hong Kong isolate from a San Francisco traveler.
Finding the same markers in Tennessee several months later supports circulation of a Tamiflu resistant swine H1N1.

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PostPosted: Tue Dec 01, 2009 10:52 am 
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Joined: Wed Aug 19, 2009 10:42 am
Posts: 27362
Location: Pittsburgh, PA USA
HA travel log

gb|CY052015.1| Influenza A virus (A/Norway/4023/2009(H1N1)) s... 42.1 0.018
gb|CY052007.1| Influenza A virus (A/Norway/3478/2009(H1N1)) s... 42.1 0.018
gb|CY051991.1| Influenza A virus (A/Norway/3059/2009(H1N1)) s... 42.1 0.018
gb|CY051987.1| Influenza A virus (A/Norway/2690/2009(H1N1)) s... 42.1 0.018
gb|CY051986.1| Influenza A virus (A/Norway/2674/2009(H1N1)) s... 42.1 0.018
gb|CY051982.1| Influenza A virus (A/Catalonia/S1761/2009(H1N1... 42.1 0.018
gb|CY051976.1| Influenza A virus (A/Catalonia/S1758/2009(H1N1... 42.1 0.018
gb|CY051964.1| Influenza A virus (A/Catalonia/S1748/2009(H1N1... 42.1 0.018
gb|CY051955.1| Influenza A virus (A/Catalonia/S1698/2009(H1N1... 42.1 0.018
gb|CY051940.1| Influenza A virus (A/Catalonia/S1674/2009(H1N1... 42.1 0.018
gb|CY051937.1| Influenza A virus (A/Catalonia/S1672/2009(H1N1... 42.1 0.018
gb|GU214058.1| Influenza A virus (A/Serbia/3547/2009(H1N1)) s... 42.1 0.018
gb|CY050319.1| Influenza A virus (A/Catalonia/S1641/2009(H1N1... 42.1 0.018
gb|CY050316.1| Influenza A virus (A/Catalonia/S1637/2009(H1N1... 42.1 0.018
gb|CY050313.1| Influenza A virus (A/Catalonia/S1632/2009(H1N1... 42.1 0.018
gb|CY050307.1| Influenza A virus (A/Catalonia/S1606/2009(H1N1... 42.1 0.018
gb|CY050304.1| Influenza A virus (A/Catalonia/S1605/2009(H1N1... 42.1 0.018
gb|CY050301.1| Influenza A virus (A/Catalonia/S1604/2009(H1N1... 42.1 0.018
dbj|AB530488.1| Influenza A virus (A/Nagasaki/HA-44/2009(H1N1... 42.1 0.018
gb|CY049742.1| Influenza A virus (A/Catalonia/S1592/2009(H1N1... 42.1 0.018
gb|CY049736.1| Influenza A virus (A/Catalonia/S1587/2009(H1N1... 42.1 0.018
gb|CY049728.1| Influenza A virus (A/Catalonia/S1545/2009(H1N1... 42.1 0.018
gb|CY049707.1| Influenza A virus (A/Catalonia/S1479/2009(H1N1... 42.1 0.018
gb|CY049704.1| Influenza A virus (A/Catalonia/S1478/2009(H1N1... 42.1 0.018
gb|CY049051.1| Influenza A virus (A/Catalonia/S1436/2009(H1N1... 42.1 0.018
gb|CY049027.1| Influenza A virus (A/Catalonia/S1379/2009(H1N1... 42.1 0.018
gb|CY049024.1| Influenza A virus (A/Catalonia/S1369/2009(H1N1... 42.1 0.018
gb|CY049018.1| Influenza A virus (A/Catalonia/S1350/2009(H1N1... 42.1 0.018
gb|CY049015.1| Influenza A virus (A/Catalonia/S1333/2009(H1N1... 42.1 0.018
gb|CY049012.1| Influenza A virus (A/Catalonia/S1331/2009(H1N1... 42.1 0.018
gb|CY049003.1| Influenza A virus (A/Catalonia/S1304/2009(H1N1... 42.1 0.018
gb|CY049000.1| Influenza A virus (A/Catalonia/S1300/2009(H1N1... 42.1 0.018
gb|CY047307.1| Influenza A virus (A/Catalonia/S1286/2009(H1N1... 42.1 0.018
gb|CY047290.1| Influenza A virus (A/Catalonia/S1273/2009(H1N1... 42.1 0.018
gb|CY047287.1| Influenza A virus (A/Catalonia/S1272/2009(H1N1... 42.1 0.018
gb|CY047278.1| Influenza A virus (A/Catalonia/S1269/2009(H1N1... 42.1 0.018
gb|CY047269.1| Influenza A virus (A/Catalonia/S1265/2009(H1N1... 42.1 0.018
gb|CY047266.1| Influenza A virus (A/Catalonia/S1260/2009(H1N1... 42.1 0.018
gb|CY047263.1| Influenza A virus (A/Catalonia/S1254/2009(H1N1... 42.1 0.018
gb|CY047257.1| Influenza A virus (A/Catalonia/S1249/2009(H1N1... 42.1 0.018
gb|CY047254.1| Influenza A virus (A/Catalonia/S1248/2009(H1N1... 42.1 0.018
gb|CY047243.1| Influenza A virus (A/Catalonia/S1237/2009(H1N1... 42.1 0.018
gb|CY047240.1| Influenza A virus (A/Catalonia/S1236/2009(H1N1... 42.1 0.018
gb|CY047230.1| Influenza A virus (A/Catalonia/S1227/2009(H1N1... 42.1 0.018
gb|CY047227.1| Influenza A virus (A/Catalonia/S1222/2009(H1N1... 42.1 0.018
gb|CY047221.1| Influenza A virus (A/Catalonia/S1215/2009(H1N1... 42.1 0.018
gb|CY047215.1| Influenza A virus (A/Catalonia/S1209/2009(H1N1... 42.1 0.018
gb|CY047203.1| Influenza A virus (A/Catalonia/S1205/2009(H1N1... 42.1 0.018
gb|CY047200.1| Influenza A virus (A/Catalonia/S1199/2009(H1N1... 42.1 0.018
gb|CY047188.1| Influenza A virus (A/Catalonia/S1187/2009(H1N1... 42.1 0.018
gb|CY047185.1| Influenza A virus (A/Catalonia/S1183/2009(H1N1... 42.1 0.018
gb|CY047182.1| Influenza A virus (A/Catalonia/S1182/2009(H1N1... 42.1 0.018
gb|CY047164.1| Influenza A virus (A/Catalonia/S1165/2009(H1N1... 42.1 0.018
gb|CY047158.1| Influenza A virus (A/Catalonia/S1161/2009(H1N1... 42.1 0.018
gb|CY047137.1| Influenza A virus (A/Catalonia/S1120/2009(H1N1... 42.1 0.018
gb|GQ485661.1| Influenza A virus (A/Almati/01/2009(H1N1)) seg... 42.1 0.018
gb|GQ457519.1| Influenza A virus (A/Changsha/78/2009(H1N1)) s... 42.1 0.018
gb|GQ457514.1| Influenza A virus (A/California/25/2009(H1N1))... 42.1 0.018
gb|GQ422381.1| Influenza A virus (A/Milan/83/2009(H1N1)) segm... 42.1 0.018
gb|GQ422380.1| Influenza A virus (A/Milan/80/2009(H1N1)) segm... 42.1 0.018
gb|GQ421203.1| Influenza A virus (A/Ancona/05/2009(H1N1)) seg... 42.1 0.018
gb|GQ387430.1| Influenza A virus (A/Catalonia/378/2009(H1N1))... 42.1 0.018
gb|GQ387382.1| Influenza A virus (A/Italy/172/2009(H1N1)) hem... 42.1 0.018
gb|GQ365446.1| Influenza A virus (A/Sapporo/1/2009(H1N1)) seg... 42.1 0.018
gb|GQ355305.1| Influenza A virus (A/Catalonia/387/2009(H1N1))... 42.1 0.018
gb|GQ351314.1| Influenza A virus (A/Hong Kong/2369/2009(H1N1)... 42.1 0.018
gb|GQ258700.1| Influenza A virus (A/Athens/893/2009(H1N1)) se... 42.1 0.018
gb|GQ249333.1| Influenza A virus (A/Paris/2591/2009(H1N1)) se... 42.1 0.018
gb|GQ160534.1| Influenza A virus (A/New Jersey/01/2009(H1N1))... 42.1 0.018

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PostPosted: Tue Dec 01, 2009 10:53 am 
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Joined: Wed Aug 19, 2009 10:42 am
Posts: 27362
Location: Pittsburgh, PA USA
dbj|AB530488.1| Influenza A virus (A/Nagasaki/HA-44/2009(H1N1... 38.2 0.19
gb|GQ457514.1| Influenza A virus (A/California/25/2009(H1N1))... 38.2 0.19
gb|GQ422381.1| Influenza A virus (A/Milan/83/2009(H1N1)) segm... 38.2 0.19
gb|GQ387430.1| Influenza A virus (A/Catalonia/378/2009(H1N1))... 38.2 0.19
gb|GQ387382.1| Influenza A virus (A/Italy/172/2009(H1N1)) hem... 38.2 0.19
gb|GQ351314.1| Influenza A virus (A/Hong Kong/2369/2009(H1N1)... 38.2 0.19

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PostPosted: Tue Dec 01, 2009 11:18 am 
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Joined: Fri Nov 13, 2009 4:17 pm
Posts: 322
niman wrote:
CDC just deposited a series of new sequences at GISAID. Several have Tamiflu resistance. On, A/Tennessee/17/2009, is the same two markers as the evolunionarily fit Hong Kong isolate from a San Francisco traveler.
Finding the same markers in Tennessee several months later supports circulation of a Tamiflu resistant swine H1N1.

It should be noted that we've had several resistance events in bordering North Carolina as well. Did those NC sequences ever get released? Seeing this "cluster" expand in this very small geographic would seem to indicate efficient spread??


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PostPosted: Tue Dec 01, 2009 11:35 am 
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Joined: Tue Aug 11, 2009 2:58 pm
Posts: 1400
Location: Katy, TX
The Mountains Voice wrote:
niman wrote:
CDC just deposited a series of new sequences at GISAID. Several have Tamiflu resistance. On, A/Tennessee/17/2009, is the same two markers as the evolunionarily fit Hong Kong isolate from a San Francisco traveler.
Finding the same markers in Tennessee several months later supports circulation of a Tamiflu resistant swine H1N1.

It should be noted that we've had several resistance events in bordering North Carolina as well. Did those NC sequences ever get released? Seeing this "cluster" expand in this very small geographic would seem to indicate efficient spread??


No, it's all randomly being generated there. Temporal and geographic relationship is not really present. Nothing to see here. Keep moving.

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PostPosted: Tue Dec 01, 2009 12:11 pm 
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Joined: Fri Nov 13, 2009 4:17 pm
Posts: 322
wotan wrote:
No, it's all randomly being generated there. Temporal and geographic relationship is not really present. Nothing to see here. Keep moving.

Do you mean they are randomly releasing sequences, or do you mean it is all spontaneous random mutation?

And when you state that temporal and geographic relationships are not present - are you implying that these are distantly old sequences just now being released? We've had report of NC resistance going well back in time, and newly just recently - so how are temporal issues ruled out? Have the NC sequences been released which prove different ancestory/travel log? Seems rather a shotgun approach to make such a conclusive generalization.


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PostPosted: Tue Dec 01, 2009 12:13 pm 
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Joined: Wed Aug 19, 2009 2:04 pm
Posts: 314
The Mountains Voice wrote:
wotan wrote:
No, it's all randomly being generated there. Temporal and geographic relationship is not really present. Nothing to see here. Keep moving.

Do you mean they are randomly releasing sequences, or do you mean it is all spontaneous random mutation?

And when you state that temporal and geographic relationships are not present - are you implying that these are distantly old sequences just now being released? We've had report of NC resistance going well back in time, and newly just recently - so how are temporal issues ruled out? Have the NC sequences been released which prove different ancestory/travel log? Seems rather a shotgun approach to make such a conclusive generalization.


I think he was joking; speaking like a beaurocrat.


Last edited by 4Peas on Tue Dec 01, 2009 12:15 pm, edited 1 time in total.

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PostPosted: Tue Dec 01, 2009 12:14 pm 
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Joined: Tue Aug 11, 2009 2:58 pm
Posts: 1400
Location: Katy, TX
The Mountains Voice wrote:
wotan wrote:
No, it's all randomly being generated there. Temporal and geographic relationship is not really present. Nothing to see here. Keep moving.

Do you mean they are randomly releasing sequences, or do you mean it is all spontaneous random mutation?

And when you state that temporal and geographic relationships are not present - are you implying that these are distantly old sequences just now being released? We've had report of NC resistance going well back in time, and newly just recently - so how are temporal issues ruled out? Have the NC sequences been released which prove different ancestory/travel log? Seems rather a shotgun approach to make such a conclusive generalization.

:wave: :wave:
Are you awake? :) That was the CDC's party line. There is no spread of Tamiflu resistance. We keep seeing H274Y coming up due to de-novo mutation within individuals. The fact that we have several cases clustered together both in time and in relatively close proximity to each other points to nothing we should be concerned about.

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I am a layman with a background in the physical sciences.


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PostPosted: Tue Dec 01, 2009 12:39 pm 
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Joined: Fri Nov 13, 2009 4:17 pm
Posts: 322
wotan wrote:
:wave: :wave:
Are you awake? :) That was the CDC's party line. There is no spread of Tamiflu resistance. We keep seeing H274Y coming up due to de-novo mutation within individuals. The fact that we have several cases clustered together both in time and in relatively close proximity to each other points to nothing we should be concerned about.

Ugghhhh! Actually, I've got "It" again - for the 2nd or 3rd time - the little beastie from which all these discussions thrive :( 11 year old had it again last week. 5 year old is getting over it now. He crawled in bed a couple nights ago and slept there hacking into my face all night. Head throbbing, throat sore, just waiting on fever / stomach to kick in. This WILL most certainly be the 3rd go around for us. 1st and 3rd had stomach issues. 2nd was more lung. This 3rd one does have more of a "Head/Nasal" component. The 1st and 3rd were more sudden and severe for the kids. I seem to be experiencing milder 3rd so far. The 2nd was LONG and low intensity drawn out miserable. Of course, it's possible one of these is something new - a "B" or something. Again... Ughhhh, I just want it gone. It was intense but passed REALLY fast for kids this time... <18 hours for 11 yr old. <36 for 5 year old (I can hear him happily destroying the house in background now). Of coure I can't resist the opportunity to "blame it all" on the symptom reducing powers of Vitamin D! LOL

Thanks Wotan - for a second I thought I had said something that had really ticked you off! You should have seen the reply I deleted :) LOL


Last edited by The Mountains Voice on Tue Dec 01, 2009 12:47 pm, edited 1 time in total.

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PostPosted: Tue Dec 01, 2009 12:45 pm 
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The Mountains Voice wrote:
Ugghhhh! Actually, I've got "It" again - .........Head throbbing......

PS... Off topic, and I should be kicked for not knowing this, but what is the general concensus on Aspirin / Ibuprofen / Tylenol? Wasn't there one or the other that MIGHT produce worse symptoms in event of cytokine? Naturally not expecting that..... but never hurt to play safe, and I MUST take something, my head is friekin exploding!


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