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PostPosted: Sat Jun 23, 2012 4:25 am 
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niman wrote:
niman wrote:
niman wrote:
Science 22 June 2012:
Vol. 336 no. 6088 pp. 1534-1541
DOI: 10.1126/science.1213362
•Report


Airborne Transmission of Influenza A/H5N1 Virus Between Ferrets

Substitution H103Y has only been found once, in combination with T156A in a duck in China (53). http://www.sciencemag.org/content/336/6088/1534.full

H107Y is in first H5N1 (from 1959) as well as low path H5 in Europe.

EPI353349 A/duck/Germany/R1789/2008 (A/H5N3) segment 4 (HA) 27.0 8.347818e+00 14/14 (100%)
EPI243076 A/chicken/Scotland/59 (A/H5N1) segment 4 (HA) 27.0 8.347818e+00 14/14 (100%)
EPI242232 A/Chicken/Scotland/59 (A/H5N1) segment 4 (HA) 27.0 8.347818e+00 14/14 (100%)
EPI63220 A/teal/Italy/3931-38/2005 (A/H5N2) segment 4 (HA) 27.0 8.347818e+00 14/14 (100%)

H107Y in low path H5 in North America
EPI353359 A/turkey/Ontario/7732/1966 (A/H5N9) segment 4 (HA) 27.0 8.347818e+00 14/14 (100%)
EPI306434 A/turkey/Wisconsin/1/1968 (A/H5N9) segment 4 (HA) 27.0 8.347818e+00 14/14 (100%)
EPI242657 A/turkey/CO/118899/1972 (A/H5N2) segment 4 (HA) 27.0 8.347818e+00 14/14 (100%)
EPI230652 A/turkey/Wisconsin/68 (A/H5N9) segment 4 (HA) 27.0 8.347818e+00 14/14 (100%)
EPI131134 A/mallard/Wisconsin/428/1975 (A/H5N1) segment 4 (HA) 27.0 8.347818e+00 14/14 (100%)
EPI131132 A/Mallard/Wisconsin/169/75 (A/H5N3) segment 4 (HA) 27.0 8.347818e+00 14/14 (100%)
EPI125600 A/turkey/MN/3397-3/1992 (A/H5N2) segment 4 (HA) 27.0 8.347818e+00 14/14 (100%)
EPI118658 A/mallard/WI/169/1975 (A/H5N3) segment 4 (HA) 27.0 8.347818e+00 14/14 (100%)

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PostPosted: Sat Jun 23, 2012 5:19 am 
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Joined: Wed Aug 19, 2009 10:42 am
Posts: 27559
Location: Pittsburgh, PA USA
niman wrote:
niman wrote:
Science 22 June 2012:
Vol. 336 no. 6088 pp. 1534-1541
DOI: 10.1126/science.1213362
•Report


Airborne Transmission of Influenza A/H5N1 Virus Between Ferrets

Substitution H103Y has only been found once, in combination with T156A in a duck in China (53). http://www.sciencemag.org/content/336/6088/1534.full

H107Y is in first H5N1 (from 1959) as well as low path H5 in Europe.

Low path swine H5 with H107Y and T160A:
LOCUS FJ461593 1695 bp cRNA linear VRL 13-APR-2009
DEFINITION Influenza A virus (A/swine/Korea/C13/2008(H5N2)) segment 4
hemagglutinin (HA) gene, complete cds.
ACCESSION FJ461593
VERSION FJ461593.1 GI:213688631
KEYWORDS .
SOURCE Influenza A virus (A/swine/Korea/C13/2008(H5N2))
ORGANISM Influenza A virus (A/swine/Korea/C13/2008(H5N2))
Viruses; ssRNA negative-strand viruses; Orthomyxoviridae;
Influenzavirus A.
REFERENCE 1 (bases 1 to 1695)
AUTHORS Lee,J.H., Pascua,P.N., Song,M.S., Baek,Y.H., Kim,C.J., Choi,H.W.,
Sung,M.H., Webby,R.J., Webster,R.G., Poo,H. and Choi,Y.K.
TITLE Isolation and genetic characterization of H5N2 influenza viruses
from pigs in Korea
JOURNAL J. Virol. 83 (9), 4205-4215 (2009)
PUBMED 19359528
REFERENCE 2 (bases 1 to 1695)
AUTHORS Lee,J.H., Pascua,P.N.Q., Oh,T.-K., Song,M.-S., Baek,Y.H. and
Choi,Y.-K.
TITLE Direct Submission
JOURNAL Submitted (11-NOV-2008) College of Medicine and Medical Research
Institute, Chungbuk National University, 12 Gaeshin-Dong
Heungduk-Ku, Cheongju City, Chungbuk 361-763, Republic of Korea
COMMENT GenBank Accession Numbers FJ461605, FJ461603, FJ461597, FJ461601,
FJ461607, FJ461599, FJ461595, FJ461593 represent sequences from the
8 segments of Influenza A virus (A/swine/Korea/C13/2008(H5N2)).
FEATURES Location/Qualifiers
source 1..1695
/organism="Influenza A virus
(A/swine/Korea/C13/2008(H5N2))"
/mol_type="viral cRNA"
/strain="A/swine/Korea/C13/2008"
/serotype="H5N2"
/isolation_source="nasal swab/lung tissue"
/host="swine"
/db_xref="taxon:574548"
/segment="4"
/country="South Korea"
/collection_date="Feb-2008"
gene 1..1695
/gene="HA"
CDS 1..1695
/gene="HA"
/codon_start=1
/product="hemagglutinin"
/protein_id="ACJ53878.1"
/db_xref="GI:213688632"
/translation="MEKIVLLFAIVSLVKSDQICIGYHANNSTEQVDTIMEKNVTVTH
AQDILEKTHNGKLCSLNGVKPLILRDCSVAGWLLGNPMCDEFLNVPEWSYIVEKDSPI
NGLCYPGDFNDYEELKYLLSSTNHFEKIQIIPRSSWSNHDASSGVSSACPYNGRSSFF
RNVVWLIKKNNAYPTIKRSYNNTNQEDLLVLWGIHHPNDAAEQTKLYQNPTTYVSVGT
STLNQRSVPEIATRPKVNGQSGRMEFFWTILKPNDAINFESNGNFIAPEYAYKIVKKG
YSAIMKSGLEYGNCNTKCQTPMGAINSSMPFHNIHPLTIGECPKYVKSDRLVLATGPR
NVPQRETRGLFGAIAGFIEGGWQGMVDGWYGYHHSNEQGSGYAADKESTQKAIDGITN
KVNSIIDKMNIQFEAVGKEFNNLERRIENLNKKMEDGFLDVWTYNAELLVLMENERTL
DFHDSNVKNLYDKVRLQLRDNAKELGNGCFEFYHKCDDECMESVRNGTYDYPQYSEEA
RLNREEISGVKLESIGTYQILSIYSTVASSLALAIMVAGLSFWMCSNGSLQCRICI"
ORIGIN
1 atggagaaaa tagtacttct ttttgcaata gtcagtctcg tcaaaagtga ccaaatttgc
61 attggttacc atgcaaacaa ctcgacagag caggttgaca caataatgga aaagaatgtt
121 actgtcactc atgcccaaga tatactggaa aagacacaca atggaaagct ctgcagtcta
181 aatggagtta agcctctcat tttgagggat tgtagcgtag ctggatggct ccttggaaac
241 cccatgtgtg atgaattcct caatgtgccg gaatggtcct acatagtgga gaaggacagc
301 ccaatcaatg gcctctgcta cccaggggat ttcaacgact atgaagagct gaaatacttg
361 ttgagcagta caaaccattt tgagaaaatt caaatcatcc ccaggagttc ttggtccaat
421 catgatgcct catcaggagt gagctccgca tgtccatata atgggaggtc ctcctttttc
481 agaaatgtgg tgtggctcat caaaaagaac aatgcatacc caacaataaa gaggagttac
541 aataatacta atcaagaaga tcttttggta ctgtggggga ttcaccatcc taatgatgca
601 gcagagcaga caaagctcta tcaaaaccca accacttacg tttctgttgg gacatcaaca
661 ctgaaccaga gatcagtccc agaaatagct accaggccca aagtaaatgg gcaaagtgga
721 agaatggagt ttttctggac aatcttaaag ccaaatgatg ctatcaattt cgagagtaat
781 gggaatttta ttgctccaga atatgcatac aaaattgtca agaaaggata ctcagcaatc
841 atgaaaagtg gactggaata tggtaactgc aacaccaagt gccaaactcc aatgggtgca
901 ataaactcca gcatgccatt tcacaacata caccctctca ccattggaga atgtcccaaa
961 tacgtgaagt cagatagatt agtccttgca acagggccca gaaatgtccc tcaaagagaa
1021 acaagaggac tatttggggc catagcaggc ttcatagaag gagggtggca aggaatggta
1081 gacggctggt atggatacca ccatagcaac gagcaaggga gtggatacgc tgcagacaaa
1141 gaatccaccc aaaaggcaat agatggaatc actaataagg tcaactcaat cattgacaaa
1201 atgaacattc agtttgaggc cgttgggaag gaatttaata acttagaaag gaggatagag
1261 aatttgaaca agaaaatgga agacgggttc ctagatgtct ggacttataa tgctgaactt
1321 ctggttctca tggaaaatga aagaacccta gactttcatg actcaaatgt caagaacctt
1381 tatgacaagg ttcgactaca gcttagggat aatgcaaagg agctgggtaa tggttgtttc
1441 gagttctatc acaaatgtga tgatgaatgt atggaaagtg taagaaacgg aacgtatgac
1501 tacccgcagt attcagaaga ggcaagatta aacagagagg aaataagtgg agtaaaattg
1561 gaatcaatag gaacctacca aatactgtca atttattcaa cagtggcaag ttccttagca
1621 ctggcaatca tggtagctgg tctatctttc tggatgtgct ccaatggatc attgcaatgc
1681 aggatttgca tttaa

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PostPosted: Mon Jun 25, 2012 8:09 am 
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Sequences discusssed at follwoing thread

viewtopic.php?f=5&t=8124

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PostPosted: Tue Jul 24, 2012 6:54 am 
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Thread on CEIRS meeting in New York

viewtopic.php?f=5&t=8160

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