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PostPosted: Fri Apr 16, 2010 7:07 am 
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The recently released H5N1 from a donkey in Egypt has the same 3 BP deletion seen in mild cases in children in Egypt. The mild nature of the infection in donkeys and children suggesst H5N1 is widespread in humans in Egypt.

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PostPosted: Fri Apr 16, 2010 7:13 am 
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http://www.jbiomedsci.com/content/pdf/1 ... -17-25.pdf

Isolation and characterization of highly pathogenic avian
influenza virus subtype H5N1 from donkeys
Ahmed S Abdel-Moneim1,2§, Ahmad E Abdel-Ghany3 , Salama AS Shany4
1 Department of Virology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-
Suef 62511 Egypt
2 Division of Virology, Department of Microbiology, College of Medicine and Medical
Sciences, Taif University, Al-Taif, Saudi Arabia
3 Department of Hygiene, Management & Zoonoses, Faculty of Veterinary Medicine,
Beni-Suef University, Beni-Suef 62511 Egypt
4 Department of Poultry Diseases, Faculty of Veterinary Medicine, Beni-Suef
University, Beni-Suef 62511 Egypt
§ Corresponding author:
E-mail: asa@bsu.edu.eg (A.S.A.)

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PostPosted: Fri Apr 16, 2010 7:14 am 
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LOCUS GU371911 1704 bp cRNA linear VRL 15-APR-2010
DEFINITION Influenza A virus (A/equine/Egypt/av1/2009(H5N1)) segment 4
hemagglutinin (HA) gene, complete cds.
ACCESSION GU371911
VERSION GU371911.1 GI:283137514
KEYWORDS .
SOURCE Influenza A virus (A/equine/Egypt/av1/2009(H5N1))
ORGANISM Influenza A virus (A/equine/Egypt/av1/2009(H5N1))
Viruses; ssRNA negative-strand viruses; Orthomyxoviridae;
Influenzavirus A.
REFERENCE 1 (bases 1 to 1704)
AUTHORS Abdel-Moneim,A.S., Abdel-Ghany,A.E. and Shany,S.A.
TITLE Isolation and characterization of highly pathogenic avian influenza
virus subtype H5N1 from donkeys
JOURNAL J. Biomed. Sci. 17, 25 (2010)
REMARK Publication Status: Online-Only
REFERENCE 2 (bases 1 to 1704)
AUTHORS Abdel-Moneim,A.S., Abdel-Ghany,A.E. and Shany,S.A.S.
TITLE Direct Submission
JOURNAL Submitted (01-JAN-2010) Faculty of Veterinary Medicine, Beni-Suef
University, Beni-Suef 62511, Egypt
FEATURES Location/Qualifiers
source 1..1704
/organism="Influenza A virus
(A/equine/Egypt/av1/2009(H5N1))"
/mol_type="viral cRNA"
/strain="A/equine/Egypt/av1/2009"
/serotype="H5N1"
/isolation_source="nasal discharge"
/host="equine"
/db_xref="taxon:703555"
/segment="4"
/lab_host="SPF embryonated chicken eggs"
/country="Egypt: Beni-Suef Governorate, Aborady Village"
/collection_date="24-Mar-2009"
gene 1..1704
/gene="HA"
CDS 1..1704
/gene="HA"
/codon_start=1
/product="hemagglutinin"
/protein_id="ADB12241.1"
/db_xref="GI:283137515"
/translation="MEKIVLLLAIVSLVKSDQICIGYHANNSTEQGDTIMEKNVTVTH
AQDILEKTHNGKLCNLDGVKPLILRDCSVAGWLLGNPMCDEFLNVPEWSYIVEKINPA
NDLCYPGNFNDYEELKHLLSRINHFEKIQIIPKNSWSDHEASGVSSACPYQGRSSFFR
NVEWLTKKDNAYPTIKRSYNNTTQEDLLVLWGIHHPNDATEQTRLYQNPTTYISVGTS
TLNQRLVPKIATRSKVNGQSGRMEFFWTILKSNDAINFESNGNFIAPENAYKIVKKGD
STIMKSELEYGNCNTKCQTPIGAINSSMPFHNIHPLTIGECPKYVKSNRLVLATGLRN
SPQGERRRKKRGLFGAIAGFIEGGWQGMVDGWYGYHHSNEQGSGYAADKESTQKAIDG
VTNKVNSIIDKMNTQFEAVGREFNNLERRIENLNKKMEDGFLDVWTYNAELLVLMENE
RTLDFHDSNVKNLYDKVRLQLRDNAKELGNGCFEFYHRCDNECMESVRNGTYDYPQYS
EEARLKREEISGVKLESIGTYQILSIYSTVTSSLALAIMVAGLFLWMCSNGSLQCRIC
I"
ORIGIN
1 atggagaaaa tagtgcttct tcttgcaata gtcagtcttg ttaaaagtga tcagatttgc
61 attggttacc atgcaaacaa ctcgacagag cagggtgaca caataatgga aaagaacgtc
121 actgttacac acgcccaaga catactggaa aagacacaca acgggaaact ctgcaatcta
181 gatggagtga agcctctaat tttaagagat tgtagtgtag ccggatggct cctcgggaac
241 ccaatgtgcg acgaattcct caatgtgccg gaatggtctt acatagtgga gaagatcaat
301 ccagccaatg acctctgtta tccagggaat ttcaacgact atgaagaact gaaacaccta
361 ttgagcagaa taaaccattt tgagaaaatt cagatcatcc ccaaaaattc ttggtcagat
421 catgaagcct caggagtgag ctcagcatgt ccataccagg gaagatcctc cttttttaga
481 aatgtggaat ggcttaccaa aaaggacaat gcatacccaa caataaagag aagttacaat
541 aataccaccc aagaagatct tttggtacta tgggggattc accacccaaa tgatgcgaca
601 gagcagacaa ggctttatca aaacccaact acctatattt ccgttgggac atcaaccctg
661 aatcagagat tggtaccaaa gatagctact agatctaagg taaacgggca aagtggaagg
721 atggagttct tttggacaat tttaaaatcg aatgatgcaa taaactttga gagtaatgga
781 aatttcattg ctccagaaaa tgcatacaaa attgtcaaga aaggggactc aacaattatg
841 aaaagtgagt tggaatatgg taactgcaac accaagtgtc agactccaat aggggcgata
901 aactccagta tgccattcca caacatccac cctctcacca tcggggaatg ccccaaatat
961 gtgaaatcaa acagattagt ccttgctact gggctcagaa atagccctca aggagagaga
1021 agaagaaaaa agagaggact atttggagct atagcaggtt ttatagaggg aggatggcag
1081 ggaatggtag atggttggta tgggtaccac catagcaacg agcaggggag tgggtacgct
1141 gcagacaaag aatccactca aaaggcaata gatggagtca ccaataaggt caactcgatc
1201 attgacaaaa tgaacactca gtttgaggct gttggaaggg aatttaataa cttagaaagg
1261 agaatagaaa atttaaacaa gaagatggaa gacggattcc tagatgtctg gacttataat
1321 gctgaacttc tggttcttat ggaaaatgag agaactctag actttcatga ctcaaatgtc
1381 aagaaccttt acgacaaggt ccgactacag cttagggata atgcaaagga gcttggtaac
1441 ggttgtttcg agttctatca cagatgtgat aatgaatgta tggaaagtgt aagaaacgga
1501 acgtatgact acccgcagta ttcagaagaa gcaagattaa aaagagagga aataagtgga
1561 gtaaaattgg agtcaatagg aacttaccaa atactgtcaa tttattcaac agtgacgagc
1621 tccctagcac tggcaatcat ggtggctggt ctatttttat ggatgtgctc caatggatcg
1681 ttacaatgca gaatttgcat ttaa

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PostPosted: Fri Apr 16, 2010 9:04 am 
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Wasn't this predicted from the number cases in Egyptian children prior to pandemic swine flu?


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PostPosted: Fri Apr 16, 2010 9:20 am 
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Dingo wrote:
Wasn't this predicted from the number cases in Egyptian children prior to pandemic swine flu?

Exactly right.

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PostPosted: Fri Apr 16, 2010 9:23 am 
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niman wrote:
Dingo wrote:
Wasn't this predicted from the number cases in Egyptian children prior to pandemic swine flu?

Exactly right.

In this study, we isolated H5N1 form donkeys clinically affected with moderate
respiratory distress including cough, fever and serous nasal discharge. The course of
the disease was short (72h) and responsive well after two shots of
streptomycin/penicillin antibiotic therapy and one shot of antipyretic (Diclofenac
sodium) with no recorded mortalities.

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PostPosted: Fri Apr 16, 2010 9:24 am 
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niman wrote:
Dingo wrote:
Wasn't this predicted from the number cases in Egyptian children prior to pandemic swine flu?

Exactly right.


For many years, I have been reading commentaries from some bearded guy in Pittsburgh......


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PostPosted: Fri Apr 16, 2010 9:25 am 
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The disease recoded on 24th March 2009, 1wk after an outbreak of
H5N1 infection in poultry in the village, where many donkeys suffered from the same
clinical manifestations in an epidemic manner. The virus was isolated from a pool of
nasal discharge from three affected animals. It produced haemagglutination only
after the 3rd egg passage. RT-PCR was performed to the full length of both NA and
HA genes, where they were sequenced directly after gel purification. Sequences
were deposited in GenBank under accession numbers; GU371911 and GU371912 for
HA and NA respectively.

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PostPosted: Fri Apr 16, 2010 9:27 am 
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niman wrote:
Dingo wrote:
Wasn't this predicted from the number cases in Egyptian children prior to pandemic swine flu?

Exactly right.

I believe mild H5N1 is WIDESPREAD in Egypt and expected it to be discovered while testing H1N1 cases. Instead, the cover was blown by donkey tests!

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PostPosted: Fri Apr 16, 2010 10:45 am 
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AVIAN INFLUENZA (24): EGYPT, DONKEY
***********************************
A ProMED-mail post
<http://www.promedmail.org>
ProMED-mail is a program of the
International Society for Infectious Diseases
<http://www.isid.org>

Date: Wed 14 Apr 2010
Source: Journal of Biomedical Science 2010, 17:25 [edited]


Isolation and characterization of highly pathogenic avian influenza
virus subtype H5N1 from donkeys - Provisional abstract
----------------------------
Highly pathogenic H5N1 is a major avian pathogen that crosses species
barriers and seriously affects humans as well as some mammals. It
mutates in an intensified manner and is considered a potential
candidate for the possible next pandemic with all the catastrophic
consequences.

Methods
------------
Nasal swabs were collected from donkeys suffering from respiratory
distress. The virus was isolated from the pooled nasal swabs in
specific pathogen free embryonated chicken eggs (SPF-ECE). Reverse
transcriptase polymerase chain reaction (RT-PCR) and sequencing of
both haemagglutingin and neuraminidase were performed. H5
seroconversion was screened using haemagglutination inhibition (HI)
assay on 105 donkey serum samples.

Results
----------
We demonstrated that H5N1 jumped from poultry to another mammalian
host, donkeys. Phylogenetic analysis showed that the virus clustered
within the lineage of H5N1 from Egypt closely related to 2009
isolates. It harboured few genetic changes compared to the closely
related viruses from avian and humans. The neuraminidase lacks
oseltamivir resistant mutations. Interestingly, HI screening for
antibodies to H5 haemagglutinins in donkeys revealed a high exposure
rate.

Conclusions
------------
These findings extend the host range of the H5N1 influenza virus,
possess implications for influenza virus epidemiology, and highlight
the need for the systematic surveillance of H5N1 in animals in the
vicinity of backyard poultry units, especially in endemic areas.

[Byline: Ahmed S Abdel-Moneim, Ahmad E Abdel-Ghany, Salama AS Shany]

--
Communicated by:
ProMED-mail <promed@promedmail.org>

[The donkey has already been added to the other 16 animals of the
class Mammalia included in the USGS List of Species affected by H5N1
(Avian Influenza), (referenced reports of highly pathogenic avian
influenza H5N1 in wildlife and domestic animals), which is available
at
<http://www.nwhc.usgs.gov/disease_information/avian_influenza/affected_species_chart.jsp>.

For the full paper from which the abstract has been copied, see:

Ref 1. Ahmed S Abdel-Moneim, Ahmad E Abdel-Ghany & Salama AS Shany
(2010). Isolation and characterization of highly pathogenic avian
influenza virus subtype H5N1 from donkeys. Journal of Biomedical
Science 2010, 17:25; doi:10.1186/1423-0127-17-25;
<http://www.jbiomedsci.com/content/pdf/1423-0127-17-25.pdf>. - Mod.AS]

[see also:
2009
----
Avian influenza (32): Japan, raccoons, serology 20090407.1348
2008
----
Undiagnosed die-off, wild birds - Mongolia: RFI 20080904.2767
Announcements 2008 (01): Global Wildlife Disease Map 20080505.1541
Avian influenza (45): Viet Nam, civet, RFI 20080313.1003
2006
----
Avian influenza (202) - Indonesia: porcine, feline 20061008.2890
2005
----
Avian influenza - Eurasia (78): wild birds 20051124.3413
Avian influenza - Asia (10): migratory birds 20050824.2492]
........................................................arn/msp/lm

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