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niman
PostPosted: Sun Dec 04, 2011 10:31 pm 
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Joined: Wed Aug 19, 2009 10:42 am
Posts: 27559
Location: Pittsburgh, PA USA
niman wrote:
gsgs wrote:
> The above data clearly shows the 2011 H3N2pdm11 PB2 evolving
> from the 2010 human trH3N2 sequences.

.., and the Sw/PA

but evolving probably not in humans.

3 (or 4) different strains, only one of them with sequences in 2010,2011

rooted to their average, not to the likely ancestor

Please. The swine in PA match A/Pennsylvania/14/2011 which is an OUTLIER!

Look at the HA sequences.

You really don't have a clue, even AFTER you have the GISAID sequences. The 2011 H3N2pdm11 sequences evolve from A/Wisconsin/12/2010 and A/Pennsylvania/40/2010, which match, but you don't even have PA/40/2010 in your table. The 2011 have NA from A/Pennsylvania/14/2010, PB1 from an earlier version similar to A/Ohio/01/2007, and an M gene from H1N1pdm09. The other five genes are from the dominant sequences from the 2010 human trH3N2 sequences.

In 2010 most of the human isoaltes match in most of the gene segemnts. In 2011 all of the human isolates match in all of the gene segments.

Slide 6 shows the clear clustering of the HA sequences from the 2010 isolates, which are the precusors for the ten 2011 H3N2pdm11 sequences. Four of the five isolates are in red and at the top of the tree on the same branch. A/Pennsylvania/14/2010 is the OUTLIER. which is on a seperate branch. The daughter of MN/11/10 would match, but confirmation of trH3N2 was by serology, so there is no sequence, but she was infected by her father.

http://www.google.com/url?sa=t&rct=j&q= ... Q8C92QA5KQ

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gsgs
PostPosted: Sun Dec 04, 2011 10:40 pm 
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Location: germany
why should it cluster in humans more than in swine ?

we had all 8 segments before just not so many viruses.
It did show frequent reassortment which is typical for swine
but rare for human flu.

who else believes it evolves in humans ?

show some mutations that were likely acquired in humans

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niman
PostPosted: Sun Dec 04, 2011 10:56 pm 
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Joined: Wed Aug 19, 2009 10:42 am
Posts: 27559
Location: Pittsburgh, PA USA
gsgs wrote:
why should it cluster in humans more than in swine ?

we had all 8 segments before just not so many viruses.
It did show frequent reassortment which is typical for swine
but rare for human flu.

who else believes it evolves in humans ?

show some mutations that were likely acquired in humans

Please. You don't have a clue. Stick with the bable boards.

The clustering in 2010 demonstrate the adaptaion to humans. In 2011 ALL human sequences have the same constellation and the reassortment is WITHIN this sub-clade, becasue that is the subclade circulating in HUMANS. The reassortment in 2011 is between HUMAN 2011 sequences. There is only ONE example in swine, because it is much more common in humans.

You really are analytically challenged. The clustering is OBVIOUS. In Nancy Cox's slide the 2010 human sequences are COLOR CODED.

You have problems with EXTREMELY clear data.

Maybe you should read more on phylogenetic analysis. These trees can be generated with PUBLIC and FREE SOFTWARE. You seem have have problems understanding the tables you post, so maybe diagrams as represented by phylogenetic trees would help.

Evolution is pretty straightforward, for most.

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gsgs
PostPosted: Mon Dec 05, 2011 12:00 am 
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Location: germany
I regrouped a bit manually: (still only PB2)


Code:
                                             00000000000000000000000000000000000000000000000000111111111111111111111111111111111111111111111111111111111122222222222 
                                             00000111111122222233333334444555666667777788899999000000111122223333333444444445555555566666677778888889999900000001222 
                                             01148011347845666722456692459017345791125846800459013689167702382235788234666780124667814467904680335590223500115899158 
                                             64728547844258146915544696735706024301730604339516510438943661610620116214147059242062474787876880131465063617098243190 
-codon-position------------------------------ 22 1      22   2  1  1            1        1       12               1   1                     1  1       1             
---Index-------------------------------------GAACAAGAAGAAAGCTTCCGTTGCCATTAATGGGCAAACGGTCGGCGGGAAAGGACTGTAGGGAAAGAGAGGTAGTCCAATATGATACCTCCAGTAGCGCATGGGACTTAATGCTGGAG 
   1 >Index0                                 ....................................................................................................................... 
   2 >A/Sw/IO/01700,2007/07/31               ...............C.T.........C.......G.........TA...........GG............G.A....G.....G.................A..T...G...C.... 
   3 >A/Sw/Index/triple-reass./1998(H3N2)    .............A.C.TT........C.....A.GGG.......TA...........GG..........A.G.AC...G.....G.....T....A......A..A...G..TCA... 
   4 >A/Sw/Ontario/33853/2005//              ...............C.T.........C.......G.........T............GG............G.A...GG.....G.....T...........A..T...G..TC.... 
   5 >A/Sw/IL/02450,2008/11/19               ..........G....C.T.........C.......G.........T............AG............G.A...GG.G...G........C........A..T...G..TC.... 
...
   6 >A/Sw/MN/02905,2009/10/21               .......................T............G.....A.......................A..........T......................................... 
   7 >A/Sw/MO/02060,2008//                   ........G......................A...........................................................T....A............G........- 
   8 >A/Sw/NC/R08-001877-D08-013371,2008//   ...............................A................................................................A....C.......G......... 
   9 >A/Sw/MN/1300,2007/01/31                ...........................C.........................................G...............................................GA 
  26 >A/KA/13/2009/07/27(H3N2)             h ..............................................A........T..........................C...................................- 
  11 >A/Sw/MN/02011,2008//                   ............................G......G..............................................C...............A...A............A..- 
  25 >A/Sw/MN/02475,2008/12/08               ............................G........G...........................G...G............C......C....CG.TA...A...T........A... 
  10 >A/Sw/MN/SG239,2007/02/23               ............................G....................................G................C..........T.G..A...A............A... 
  12 >A/Sw/MN/578,2007/01/24                 ..................T.........G......G....A.........................................C...................................A 
  24 >A/Sw/MN/01862,2007/11/20               .G..............C...........G.....................G...............A.............C.C..............T...C...G....G....AA.. 
...
  13 >A/Sw/IL/01917,2007/12/07               .G........G...T.C...........G......G..............G....T..........A...........G.C.C..............T.......G............. 
  14 >A/Sw/IL/01918,2007/12/07               .G........G...T.C...........G......G..............G....T..........A...........G.C.C..............T.......G............. 
  15 >A/Sw/IL/01981,2008/01/16               .G........G...T.C...........G......G..............G....T..........A...........G.C.C..............T.......G............. 
  20 >A/Sw/IL/Sg-00445,2008/01/16            .G........G...T.C...........G....A.G..............G....T..........A...........G.C.C..............T.......G............. 
  16 >A/Sw/IL/02238,2008/07/16               .G........G...T.............G......G..............G....T..........A...........G.C.C.....T........T.......G............. 
  17 >A/Sw/IL/Sg-00443,2008/07/16            .G........G...T.............G......G..............G....T..........A...........G.C.C.....T........T.......G............. 
  18 >A/Sw/IL/02251,2008/07/16               .G..G.....G...T.............G......G......A.......G....T..........A...........G.C.C.....T........T.......G............. 
  19 >A/Sw/IL/Sg-00444,2008/07/16            .G..G.....G...T.............G......G......A.......G....T..........A...........G.C.C.....T........T.......G............. 
  21 >A/Sw/IL/02510,2009/01/12               .G........G...T....A.C......G......G............T.G....T.....A....A...........G.C.C.....T........T.......G.....C....... 
  22 >A/Sw/IL/02695,2009/03/31               .G........G...T....A.C......G......G............T.G....T.....A....A...........G.C.C.....T........T.......G.....C....... 
  23 >A/Sw/IL/02385,2008/10/17               .G........G...T....A........G......G............T.G....T.....A....A..G........G.C.C.....T........T.......G.....C....... 
...
  37 >A/Michigan/09/2007/08/19(H1N2)       h .................................................G.T................A.............C.................................... 
  27 >Index1                                 .................................................G.T..G.............A.A...........C.................................... 
  28 >A/Sw/IL/SG-00244,2007/07/31            --...............................................G.T..G.............A.A...........C........................C..........- 
  29 >A/Sw/OH/24366,2007//                   .................................................G.T..G.....T.......A.A...........C........................C..........- 
  30 >A/Ohio/01/2007/08/17(H1N1) 36f       h .................................................G.T..G.....T.......A.A...........C........................C........... 
  31 >A/Ohio/02/2007/08/17(H1N1)  10f      h .................................................G.T..G.....T.......A.A...........C........................C........... 
  32 >A/Sw/OH/511445,2007//                  ..G..............................................G.T..G.....T.......A.A...........C........................C........... 
  33 >A/Sw/OH/FAH1-1,2007//                  ........G........................................G.T..G.....T.......A.A....C......C........................C..........- 
  34 >A/Illinois/09/2007(H1N1) (NA not)    h .................................................G.T..G.............A.A...........C..........T.............C........... 
  35 >A/Sw//OH/02026,2008/02/15              .................................................G.T..G.............A.A...........CA.........A.............C........... 
  36 >A/Sw/KA/77778,2007/12/                 .................................................G.TA.G.............A.A..........GC.......T................C.G..T...... 
  38 >A/Sw/SD/152B,2009/09/04                .....................C...........................G.TA.G........G....A.A..........GC......CT.....T..........C.G..T...... 
...
  39 >A/Sw/PA/057108-1,2010/09/20            ..G...A..........................................G.TA.G.........G..GA.A..CA..T...GC.......T.G......T.......C.........G. 
...
  56 >A/Sw/MN/02782,2009/05/07               .G..................C....G......A......AA..A.....G.T..G.........G...A.A...A.......CAG......................C.......AA.. 
  40 >A/Sw/PA/62170-4/2010/10/25             .....G.G...G........C....G......A.T......C.AA....G.T..G.........G..GA.A..CA..T....C.G.......G......T.......CC........G. 
  41 >A/Sw/PA/62170-3,2010/10/25             .....G.G...G........C....G......A.T......C.AA....G.T..G.........G..GA.A...A..T....C.G.......G......T.......CC........G. 
  42 >A/Sw/PA/62170-1/2010/10/25             .....G.G...G........C....G......A.T......C.AA....G.T..G....G....G..GA.A...A..T....C.G.......G......T.......CC........G. 
  43 >A/Sw/PA/62170-2/2010/10/25             .....G.G...G........C....G......A.T......C.AA....G.T..G....G....G..GA.A...A..T....C.G.......G......T.......CC........G. 
  44 >A/PA/14/2010/10/26(H1N1)             h .....G.G...G........C....G......A.T......C.AA....G.T..G.........G..GA.A...A..T....C.G.......G......T.......CC.......... 
  45 >A/ME/06/2011/10/10                   h .....G.G...G.A......CC...G......A.T......C.AA....G.T..G.........G..GA.A...A..T....C.G.......G......T.......CC........G- 
  46 >A/Wisconsin/12/2010/11/02(H3N2)      h .......G...G........C........T..A.T....A.C.AA....G.T.AG.........G...A.A...A..T...GC.G.......G......T.......C.........G. 
  47 >A/Sw/MN/A01047613,2010/12/10           .......G...G........C........T..A.T....A.C.AA....G.T.AG.........G...A.A...A......GC.G.......G......T.......C.........G. 
  53 >A/IN/08_a/2011/07/27                 h ...T.GAG.A.GC.......C.....C...C.A.T...TA.C.AA....G.T..G..A....AGG..GA.AA..A.AT....C.G.C.....G......TG...A............G. 
  54 >A/IN/08_b/2011/07/27                 h ...T.GAG.A.GC.......C.....C...C.A.T...TA.C.AA....G.T..G..A....AGG..GA.AA..A.AT....C.G.C.....G......TG...A............-- 
  55 >A/PA/09/2011/08/20                   h ...T.G.G.A.GC.......C.....C...C.A.T...TA.C.AA....G.T..G..A....AGG..GA.AA..A.AT....C.G.C.....G......TG...A............G. 
  48 >A/Sw/NY/A01104005/2011/09/13           ...T.G.G...GC.......C...T.C...C.A.T...TA.C.AA....G.T..G..A.....GG..GA.AA..A..T....C.G.C.....G......T....A............G. 
  49 >A/IN/10/2011/10/22                   h ...T.G.G...GC.......C..TT.C...C.A.T...TA.C.AA....G.T..G..A.....GG..GA.AA..A..T....C.G.C.....G......T....A............G- 
  50 >A/Iowa/07(f3)/2011/11/14(H3N2)       h A..T.G.G...GC.......C.A.T.C...C.A.T...TA.C.AA..A.G.T..G.CA.....GG..GA.AA..A..T....C.G.CA....G......T....A............-- 
  51 >A/Iowa/08(m1)/2011/11/14(H3N2)       h A..T.G.G...GC.......C.A.T.C...C.A.T...TA.C.AA..A.G.T..G.CA.....GG..GA.AA..A..T....C.G.CA....G......T....A............-- 
  52 >A/Iowa/09(m2)/2011/11/14(H3N2)       h A..T.G.G...GC.......C.A.T.C...C.A.T...TA.C.AA..A.G.T..G.CA.....GG..GA.AA..A..T....C.G.CA....G......T....A............-- 
                                                                                                                                                                    
---Index-------------------------------------GAACAAGAAGAAAGCTTCCGTTGCCATTAATGGGCAAACGGTCGGCGGGAAAGGACTGTAGGGAAAGAGAGGTAGTCCAATATGATACCTCCAGTAGCGCATGGGACTTAATGCTGGAG 
-codon-position------------------------------ 22 1      22   2  1  1            1        1       12               1   1                     1  1       1             
                                             00000000000000000000000000000000000000000000000000111111111111111111111111111111111111111111111111111111111122222222222 
                                             00000111111122222233333334444555666667777788899999000000111122223333333444444445555555566666677778888889999900000001222 
                                             01148011347845666722456692459017345791125846800459013689167702382235788234666780124667814467904680335590223500115899158 
                                             64728547844258146915544696735706024301730604339516510438943661610620116214147059242062474787876880131465063617098243190 
.
.


.
.

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niman
PostPosted: Mon Dec 05, 2011 12:42 am 
Online

Joined: Wed Aug 19, 2009 10:42 am
Posts: 27559
Location: Pittsburgh, PA USA
gsgs wrote:
I regrouped a bit manually: (still only PB2)


Code:
                                             00000000000000000000000000000000000000000000000000111111111111111111111111111111111111111111111111111111111122222222222 
                                             00000111111122222233333334444555666667777788899999000000111122223333333444444445555555566666677778888889999900000001222 
                                             01148011347845666722456692459017345791125846800459013689167702382235788234666780124667814467904680335590223500115899158 
                                             64728547844258146915544696735706024301730604339516510438943661610620116214147059242062474787876880131465063617098243190 
-codon-position------------------------------ 22 1      22   2  1  1            1        1       12               1   1                     1  1       1             
---Index-------------------------------------GAACAAGAAGAAAGCTTCCGTTGCCATTAATGGGCAAACGGTCGGCGGGAAAGGACTGTAGGGAAAGAGAGGTAGTCCAATATGATACCTCCAGTAGCGCATGGGACTTAATGCTGGAG 
   1 >Index0                                 ....................................................................................................................... 
   2 >A/Sw/IO/01700,2007/07/31               ...............C.T.........C.......G.........TA...........GG............G.A....G.....G.................A..T...G...C.... 
   3 >A/Sw/Index/triple-reass./1998(H3N2)    .............A.C.TT........C.....A.GGG.......TA...........GG..........A.G.AC...G.....G.....T....A......A..A...G..TCA... 
   4 >A/Sw/Ontario/33853/2005//              ...............C.T.........C.......G.........T............GG............G.A...GG.....G.....T...........A..T...G..TC.... 
   5 >A/Sw/IL/02450,2008/11/19               ..........G....C.T.........C.......G.........T............AG............G.A...GG.G...G........C........A..T...G..TC.... 
...
   6 >A/Sw/MN/02905,2009/10/21               .......................T............G.....A.......................A..........T......................................... 
   7 >A/Sw/MO/02060,2008//                   ........G......................A...........................................................T....A............G........- 
   8 >A/Sw/NC/R08-001877-D08-013371,2008//   ...............................A................................................................A....C.......G......... 
   9 >A/Sw/MN/1300,2007/01/31                ...........................C.........................................G...............................................GA 
  26 >A/KA/13/2009/07/27(H3N2)             h ..............................................A........T..........................C...................................- 
  11 >A/Sw/MN/02011,2008//                   ............................G......G..............................................C...............A...A............A..- 
  25 >A/Sw/MN/02475,2008/12/08               ............................G........G...........................G...G............C......C....CG.TA...A...T........A... 
  10 >A/Sw/MN/SG239,2007/02/23               ............................G....................................G................C..........T.G..A...A............A... 
  12 >A/Sw/MN/578,2007/01/24                 ..................T.........G......G....A.........................................C...................................A 
  24 >A/Sw/MN/01862,2007/11/20               .G..............C...........G.....................G...............A.............C.C..............T...C...G....G....AA.. 
...
  13 >A/Sw/IL/01917,2007/12/07               .G........G...T.C...........G......G..............G....T..........A...........G.C.C..............T.......G............. 
  14 >A/Sw/IL/01918,2007/12/07               .G........G...T.C...........G......G..............G....T..........A...........G.C.C..............T.......G............. 
  15 >A/Sw/IL/01981,2008/01/16               .G........G...T.C...........G......G..............G....T..........A...........G.C.C..............T.......G............. 
  20 >A/Sw/IL/Sg-00445,2008/01/16            .G........G...T.C...........G....A.G..............G....T..........A...........G.C.C..............T.......G............. 
  16 >A/Sw/IL/02238,2008/07/16               .G........G...T.............G......G..............G....T..........A...........G.C.C.....T........T.......G............. 
  17 >A/Sw/IL/Sg-00443,2008/07/16            .G........G...T.............G......G..............G....T..........A...........G.C.C.....T........T.......G............. 
  18 >A/Sw/IL/02251,2008/07/16               .G..G.....G...T.............G......G......A.......G....T..........A...........G.C.C.....T........T.......G............. 
  19 >A/Sw/IL/Sg-00444,2008/07/16            .G..G.....G...T.............G......G......A.......G....T..........A...........G.C.C.....T........T.......G............. 
  21 >A/Sw/IL/02510,2009/01/12               .G........G...T....A.C......G......G............T.G....T.....A....A...........G.C.C.....T........T.......G.....C....... 
  22 >A/Sw/IL/02695,2009/03/31               .G........G...T....A.C......G......G............T.G....T.....A....A...........G.C.C.....T........T.......G.....C....... 
  23 >A/Sw/IL/02385,2008/10/17               .G........G...T....A........G......G............T.G....T.....A....A..G........G.C.C.....T........T.......G.....C....... 
...
  37 >A/Michigan/09/2007/08/19(H1N2)       h .................................................G.T................A.............C.................................... 
  27 >Index1                                 .................................................G.T..G.............A.A...........C.................................... 
  28 >A/Sw/IL/SG-00244,2007/07/31            --...............................................G.T..G.............A.A...........C........................C..........- 
  29 >A/Sw/OH/24366,2007//                   .................................................G.T..G.....T.......A.A...........C........................C..........- 
  30 >A/Ohio/01/2007/08/17(H1N1) 36f       h .................................................G.T..G.....T.......A.A...........C........................C........... 
  31 >A/Ohio/02/2007/08/17(H1N1)  10f      h .................................................G.T..G.....T.......A.A...........C........................C........... 
  32 >A/Sw/OH/511445,2007//                  ..G..............................................G.T..G.....T.......A.A...........C........................C........... 
  33 >A/Sw/OH/FAH1-1,2007//                  ........G........................................G.T..G.....T.......A.A....C......C........................C..........- 
  34 >A/Illinois/09/2007(H1N1) (NA not)    h .................................................G.T..G.............A.A...........C..........T.............C........... 
  35 >A/Sw//OH/02026,2008/02/15              .................................................G.T..G.............A.A...........CA.........A.............C........... 
  36 >A/Sw/KA/77778,2007/12/                 .................................................G.TA.G.............A.A..........GC.......T................C.G..T...... 
  38 >A/Sw/SD/152B,2009/09/04                .....................C...........................G.TA.G........G....A.A..........GC......CT.....T..........C.G..T...... 
...
  39 >A/Sw/PA/057108-1,2010/09/20            ..G...A..........................................G.TA.G.........G..GA.A..CA..T...GC.......T.G......T.......C.........G. 
...
  56 >A/Sw/MN/02782,2009/05/07               .G..................C....G......A......AA..A.....G.T..G.........G...A.A...A.......CAG......................C.......AA.. 
  40 >A/Sw/PA/62170-4/2010/10/25             .....G.G...G........C....G......A.T......C.AA....G.T..G.........G..GA.A..CA..T....C.G.......G......T.......CC........G. 
  41 >A/Sw/PA/62170-3,2010/10/25             .....G.G...G........C....G......A.T......C.AA....G.T..G.........G..GA.A...A..T....C.G.......G......T.......CC........G. 
  42 >A/Sw/PA/62170-1/2010/10/25             .....G.G...G........C....G......A.T......C.AA....G.T..G....G....G..GA.A...A..T....C.G.......G......T.......CC........G. 
  43 >A/Sw/PA/62170-2/2010/10/25             .....G.G...G........C....G......A.T......C.AA....G.T..G....G....G..GA.A...A..T....C.G.......G......T.......CC........G. 
  44 >A/PA/14/2010/10/26(H1N1)             h .....G.G...G........C....G......A.T......C.AA....G.T..G.........G..GA.A...A..T....C.G.......G......T.......CC.......... 
  45 >A/ME/06/2011/10/10                   h .....G.G...G.A......CC...G......A.T......C.AA....G.T..G.........G..GA.A...A..T....C.G.......G......T.......CC........G- 
  46 >A/Wisconsin/12/2010/11/02(H3N2)      h .......G...G........C........T..A.T....A.C.AA....G.T.AG.........G...A.A...A..T...GC.G.......G......T.......C.........G. 
  47 >A/Sw/MN/A01047613,2010/12/10           .......G...G........C........T..A.T....A.C.AA....G.T.AG.........G...A.A...A......GC.G.......G......T.......C.........G. 
  53 >A/IN/08_a/2011/07/27                 h ...T.GAG.A.GC.......C.....C...C.A.T...TA.C.AA....G.T..G..A....AGG..GA.AA..A.AT....C.G.C.....G......TG...A............G. 
  54 >A/IN/08_b/2011/07/27                 h ...T.GAG.A.GC.......C.....C...C.A.T...TA.C.AA....G.T..G..A....AGG..GA.AA..A.AT....C.G.C.....G......TG...A............-- 
  55 >A/PA/09/2011/08/20                   h ...T.G.G.A.GC.......C.....C...C.A.T...TA.C.AA....G.T..G..A....AGG..GA.AA..A.AT....C.G.C.....G......TG...A............G. 
  48 >A/Sw/NY/A01104005/2011/09/13           ...T.G.G...GC.......C...T.C...C.A.T...TA.C.AA....G.T..G..A.....GG..GA.AA..A..T....C.G.C.....G......T....A............G. 
  49 >A/IN/10/2011/10/22                   h ...T.G.G...GC.......C..TT.C...C.A.T...TA.C.AA....G.T..G..A.....GG..GA.AA..A..T....C.G.C.....G......T....A............G- 
  50 >A/Iowa/07(f3)/2011/11/14(H3N2)       h A..T.G.G...GC.......C.A.T.C...C.A.T...TA.C.AA..A.G.T..G.CA.....GG..GA.AA..A..T....C.G.CA....G......T....A............-- 
  51 >A/Iowa/08(m1)/2011/11/14(H3N2)       h A..T.G.G...GC.......C.A.T.C...C.A.T...TA.C.AA..A.G.T..G.CA.....GG..GA.AA..A..T....C.G.CA....G......T....A............-- 
  52 >A/Iowa/09(m2)/2011/11/14(H3N2)       h A..T.G.G...GC.......C.A.T.C...C.A.T...TA.C.AA..A.G.T..G.CA.....GG..GA.AA..A..T....C.G.CA....G......T....A............-- 
                                                                                                                                                                    
---Index-------------------------------------GAACAAGAAGAAAGCTTCCGTTGCCATTAATGGGCAAACGGTCGGCGGGAAAGGACTGTAGGGAAAGAGAGGTAGTCCAATATGATACCTCCAGTAGCGCATGGGACTTAATGCTGGAG 
-codon-position------------------------------ 22 1      22   2  1  1            1        1       12               1   1                     1  1       1             
                                             00000000000000000000000000000000000000000000000000111111111111111111111111111111111111111111111111111111111122222222222 
                                             00000111111122222233333334444555666667777788899999000000111122223333333444444445555555566666677778888889999900000001222 
                                             01148011347845666722456692459017345791125846800459013689167702382235788234666780124667814467904680335590223500115899158 
                                             64728547844258146915544696735706024301730604339516510438943661610620116214147059242062474787876880131465063617098243190 
.
.


.
.

Still no A/Pennsylvania/40/2010 which matches A/Wisconsin/12/2010.

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