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D225G in Chihuahua Mexico

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niman

Post subject: D225G in Chihuahua Mexico

Posted: Sat Aug 13, 2011 8:36 am

Joined: Wed Aug 19, 2009 10:42 am
Posts: 27353
Location: Pittsburgh, PA USA

The CDC has released a new series of H1N1 sequences from recent cases. Included was A/Mexico/2208/2011, which was from a 36M and collected on March 15, 2011. Those demographics matched A/Mexico/InDRE1945/2011, which had D225N (36M collected in March, 2011). However, since the latest sequence from the CDC has a different number, A/Mexico/2208/2011, may be the index case for the outbreak (when the CDC sequenced an isolate from 29M, A/Mexico/InDRE1946/2011, it was designated A/Mexico/1946/2011 by the CDC).

A/Mexico/2208/2011 was isolated in eggs and it had D225G.

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niman

Post subject: Re: D225G in Chihuahua Mexico

Posted: Sat Aug 13, 2011 9:00 am

Joined: Wed Aug 19, 2009 10:42 am
Posts: 27353
Location: Pittsburgh, PA USA

niman wrote:

At the protein level, A/Mexico/2208/2011 matches A/Mexico/InDRE1945/2011, except it has K214E and D225G.

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niman

Post subject: Re: D225G in Chihuahua Mexico

Posted: Sat Aug 13, 2011 9:08 am

Joined: Wed Aug 19, 2009 10:42 am
Posts: 27353
Location: Pittsburgh, PA USA

niman wrote:

At the protein level, A/Mexico/2208/2011 matches A/Mexico/InDRE1945/2011, except it has K214E and D225G.

A/Mexico/InDRE1945/2011 was from a diect sequence of a nasopharyngeal swab, while A/Mexico/2208/2011 was an egg isolate.

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niman

Post subject: Re: D225G in Chihuahua Mexico

Posted: Sat Aug 13, 2011 9:22 am

Joined: Wed Aug 19, 2009 10:42 am
Posts: 27353
Location: Pittsburgh, PA USA

NA also has P327S (N2 numbering).

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neuromedia

Post subject: Re: D225G in Chihuahua Mexico

Posted: Sat Aug 13, 2011 9:33 am

Joined: Wed Aug 26, 2009 4:15 pm
Posts: 623

niman wrote:

At the protein level, A/Mexico/2208/2011 matches A/Mexico/InDRE1945/2011, except it has K214E and D225G.

A/Mexico/InDRE1945/2011 was from a diect sequence of a nasopharyngeal swab, while A/Mexico/2208/2011 was an egg isolate.

Dr Niman, could both sequences* come from the very same 36M isolate ?

A numer of pH1N1 sequences were discussed in the past which displayed D225G
only when grown in eggs (but not in MDCK culture).

* sequenes: A/Mexico/2208/2011 and A/Mexico/InDRE1945/2011

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niman

Post subject: Re: D225G in Chihuahua Mexico

Posted: Sat Aug 13, 2011 10:13 am

Joined: Wed Aug 19, 2009 10:42 am
Posts: 27353
Location: Pittsburgh, PA USA

neuromedia wrote:

niman wrote:

At the protein level, A/Mexico/2208/2011 matches A/Mexico/InDRE1945/2011, except it has K214E and D225G.

A/Mexico/InDRE1945/2011 was from a diect sequence of a nasopharyngeal swab, while A/Mexico/2208/2011 was an egg isolate.

The inital sequence generated by the national labs in Mexico were a direct sequence (no isolate involved). The CDC was from an egg isolate, which selects for isolates that have increased binding activity for gal 2.3 receptors (which are on checken embryos as well as human lung).

In Ukraine, direct sequencing of autopsy lung frequently detected D225N, D225G, or both.

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neuromedia

Post subject: Re: D225G in Chihuahua Mexico

Posted: Sat Aug 13, 2011 10:41 am

Joined: Wed Aug 26, 2009 4:15 pm
Posts: 623

niman wrote:

Thank you for the explanation, Dr Niman.

D225G was frequently linked to severe cases due to such binding affinity to lung tissues.

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niman

Post subject: Re: D225G in Chihuahua Mexico

Posted: Sat Aug 13, 2011 11:29 am

Joined: Wed Aug 19, 2009 10:42 am
Posts: 27353
Location: Pittsburgh, PA USA

A800G

EPI331210 A/Mexico/2208/2011 (A/H1N1 swl) segment 4 (HA) 27.0 6.857428e+00 14/14 (100%)
EPI330172 A/Chongqing-Wanzhou/SWL53/2011 (A/H1N1 swl) segment 4 (HA) 27.0 6.857428e+00 14/14 (100%)
EPI324888 A/Quebec/QC153705/2009 (A/H1N1) segment 4 (HA) 27.0 6.857428e+00 14/14 (100%)
EPI323024 A/swine/Ohio/02195/2008 (A/H1N1) segment 4 (HA) 27.0 6.857428e+00 14/14 (100%)
EPI322836 A/swine/North Carolina/01071/2006 (A/H1N1) segment 4 (HA) 27.0 6.857428e+00 14/14 (100%)
EPI322835 A/swine/North Carolina/01070/2006 (A/H1N1) segment 4 (HA) 27.0 6.857428e+00 14/14 (100%)
EPI322612 A/swine/Nebraska/01182/2006 (A/H1N1) segment 4 (HA) 27.0 6.857428e+00 14/14 (100%)
EPI322569 A/swine/Missouri/02043/2008 (A/H1) segment 4 (HA) 27.0 6.857428e+00 14/14 (100%)
EPI322554 A/swine/Missouri/01473/2007 (A/H1N1) segment 4 (HA) 27.0 6.857428e+00 14/14 (100%)
EPI322548 A/swine/Missouri/01204/2006 (A/H1N1) segment 4 (HA) 27.0 6.857428e+00 14/14 (100%)
EPI322198 A/swine/Minnesota/00869/2005 (A/H1N1) segment 4 (HA) 27.0 6.857428e+00 14/14 (100%)
EPI322189 A/swine/Minnesota/00774/2005 (A/H1) segment 4 (HA) 27.0 6.857428e+00 14/14 (100%)
EPI322170 A/swine/Minnesota/00561/2005 (A/H1) segment 4 (HA) 27.0 6.857428e+00 14/14 (100%)
EPI322164 A/swine/Minnesota/00500/2005 (A/H1) segment 4 (HA) 27.0 6.857428e+00 14/14 (100%)
EPI322152 A/swine/Minnesota/00369/2004 (A/H1) segment 4 (HA) 27.0 6.857428e+00 14/14 (100%)
EPI322151 A/swine/Minnesota/00367/2004 (A/H1N1) segment 4 (HA) 27.0 6.857428e+00 14/14 (100%)
EPI322134 A/swine/Minnesota/00206/2003 (A/H1N1) segment 4 (HA) 27.0 6.857428e+00 14/14 (100%)
EPI322129 A/swine/Minnesota/00159/2003 (A/H1) segment 4 (HA) 27.0 6.857428e+00 14/14 (100%)
EPI322128 A/swine/Minnesota/00128/2003 (A/H1) segment 4 (HA) 27.0 6.857428e+00 14/14 (100%)
EPI322103 A/swine/Manitoba/01093/2006 (A/H1N1) segment 4 (HA) 27.0 6.857428e+00 14/14 (100%)
EPI322102 A/swine/Manitoba/01004/2006 (A/H1N2) segment 4 (HA) 27.0 6.857428e+00 14/14 (100%)
EPI321975 A/swine/Iowa/00843/2005 (A/H1N1) segment 4 (HA) 27.0 6.857428e+00 14/14 (100%)
EPI321972 A/swine/Iowa/00807/2005 (A/H1) segment 4 (HA) 27.0 6.857428e+00 14/14 (100%)
EPI321968 A/swine/Iowa/00694/2006 (A/H1N1) segment 4 (HA) 27.0 6.857428e+00 14/14 (100%)
EPI321848 A/swine/Illinois/01149/2006 (A/H1N2) segment 4 (HA) 27.0 6.857428e+00 14/14 (100%)
EPI320591 A/Lebanon/09L-12/2009 (A/H1N1 swl) segment 4 (HA) 27.0 6.857428e+00 14/14 (100%)
EPI320095 A/New Mexico/07/2011 (A/H1N1 swl) segment 4 (HA) 27.0 6.857428e+00 14/14 (100%)
EPI320093 A/New Mexico/06/2011 (A/H1N1 swl) segment 4 (HA) 27.0 6.857428e+00 14/14 (100%)
EPI320085 A/New Mexico/05/2011 (A/H1N1 swl) segment 4 (HA) 27.0 6.857428e+00 14/14 (100%)
EPI320077 A/New Mexico/04/2011 (A/H1N1 swl) segment 4 (HA) 27.0 6.857428e+00 14/14 (100%)
EPI319526 A/Trieste/12/2011 (A/H1N1 swl) segment 4 (HA) 27.0 6.857428e+00 14/14 (100%)
EPI319523 A/Trieste/04/2011 (A/H1N1 swl) segment 4 (HA) 27.0 6.857428e+00 14/14 (100%)
EPI316360 A/Indiana/05/2011 (A/H1N1 swl) segment 4 (HA) 27.0 6.857428e+00 14/14 (100%)
EPI315840 A/Mexico/InDRE1947/2011 (A/H1N1) segment 4 (HA) 27.0 6.857428e+00 14/14 (100%)
EPI312620 A/swine/Hong Kong/1112/2007 (A/H1N2) segment 4 (HA) 27.0 6.857428e+00 14/14 (100%)
EPI310249 A/HuZhou/01/2010 (A/H1N1 swl) segment 4 (HA) 27.0 6.857428e+00 14/14 (100%)
EPI308740 A/Los Angeles/INS423/2010 (A/H1N1) segment 4 (HA) 27.0 6.857428e+00 14/14 (100%)
EPI307452 A/Thailand/CU-H2085/2010 (A/H1N1) segment 4 (HA) 27.0 6.857428e+00 14/14 (100%)
EPI291696 A/Nebraska/01/1992 (A/H1N1) segment 4 (HA) 27.0 6.857428e+00 14/14 (100%)
EPI291688 A/Minnesota/18/1995 (A/H1N1) segment 4 (HA) 27.0 6.857428e+00 14/14 (100%)
EPI291680 A/Indiana/05/1995 (A/H1N1) segment 4 (HA) 27.0 6.857428e+00 14/14 (100%)
EPI291672 A/Indiana/01/1994 (A/H1N1) segment 4 (HA) 27.0 6.857428e+00 14/14 (100%)
EPI288330 A/Boston/703/2009 (A/H1N1) segment 4 (HA) 27.0 6.857428e+00 14/14 (100%)
EPI287761 A/Athens/INS393/2010 (A/H1N1) segment 4 (HA) 27.0 6.857428e+00 14/14 (100%)
EPI282143 A/New York/INS432/2010 (A/H1N1) segment 4 (HA) 27.0 6.857428e+00 14/14 (100%)
EPI281798 A/Utah/AF2653/2010 (A/H1N1) segment 4 (HA) 27.0 6.857428e+00 14/14 (100%)
EPI257808 A/Shanghai-Luwan/SWL157/2010 (A/H1N1 swl) segment 4 (HA) 27.0 6.857428e+00 14/14 (100%)
EPI183376 A/Paris/2670/2009 (A/H1N1 swl) segment 4 (HA) 27.0 6.857428e+00 14/14 (100%)
EPI178952 A/Maryland/12/1991 (A/H1N1 seasonal) segment 4 (HA) 27.0 6.857428e+00 14/14 (100%)
EPI172963 A/swine/Ratchaburi/NIAH550/2003 (A/H1N1) segment 4 (HA) 27.0 6.857428e+00 14/14 (100%)
EPI131098 A/swine/England/283902/93 (A/H1N1) segment 4 (HA) 27.0 6.857428e+00 14/14 (100%)
EPI131094 A/swine/England/117316/86 (A/H1N1) segment 4 (HA) 27.0 6.857428e+00 14/14 (100%)
EPI129192 A/MD/12/1991 (A/H1N1) segment 4 (HA) 27.0 6.857428e+00 14/14 (100%)
EPI6763 A/Swine/Wisconsin/457/98 (A/H1N1) segment 4 (HA) 27.0 6.857428e+00 14/14 (100%)
EPI6757 A/Swine/Wisconsin/168/97 (A/H1N1) segment 4 (HA) 27.0 6.857428e+00 14/14 (100%)
EPI6755 A/Swine/Wisconsin/166/97 (A/H1N1) segment 4 (HA) 27.0 6.857428e+00 14/14 (100%)
EPI6753 A/Swine/Wisconsin/164/97 (A/H1N1) segment 4 (HA) 27.0 6.857428e+00 14/14 (100%)
EPI6751 A/Swine/Wisconsin/163/97 (A/H1N1) segment 4 (HA) 27.0 6.857428e+00 14/14 (100%)

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niman

Post subject: Re: D225G in Chihuahua Mexico

Posted: Sat Aug 13, 2011 11:32 am

Joined: Wed Aug 19, 2009 10:42 am
Posts: 27353
Location: Pittsburgh, PA USA

niman wrote:

Note that A800G is in one of the other early isolates from Mexico, as well as rare isolates from New Mexico. These are NOT coincidences.

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niman

Post subject: Re: D225G in Chihuahua Mexico

Posted: Wed Aug 17, 2011 3:04 pm

Joined: Wed Aug 19, 2009 10:42 am
Posts: 27353
Location: Pittsburgh, PA USA

S165N update

EPI332607 A/Alaska/14/2011 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI331210 A/Mexico/2208/2011 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI330992 A/Mexico/1946/2011 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI330986 A/California/21/2011 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI324470 A/South Carolina/NHRC0002/2011 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI324279 A/swine/QC/1036/2009 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI324278 A/swine/QC/729-B/2009 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI324277 A/swine/QC/F8154/2009 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI324276 A/swine/QC/3193/2009 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI324131 A/swine/QC/4150/2009 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI324128 A/swine/QC/4113/2009 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI324125 A/swine/QC/4112/2009 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI324122 A/swine/QC/4110/2009 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI324119 A/swine/QC/4015-2/2009 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI324116 A/swine/QC/4015-1/2009 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI324113 A/swine/QC/3845/2009 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI324110 A/swine/QC/3777/2009 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI324107 A/swine/QC/3639-4/2009 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI324104 A/swine/QC/3639-3/2009 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI324101 A/swine/QC/3453/2009 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI324096 A/swine/QC/3399-6/2009 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI324090 A/swine/QC/3399-3/2009 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI324087 A/swine/QC/1814-5/2009 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI324084 A/swine/QC/1814-3/2009 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI324081 A/swine/QC/1814-1/2009 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI324078 A/swine/QC/1805-5/2009 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI324075 A/swine/QC/1805-4/2009 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI324072 A/swine/QC/1805-3/2009 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI324069 A/swine/QC/1805-2/2009 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI324066 A/swine/QC/1805-1/2009 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI324063 A/swine/QC/1792-2/2009 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI324060 A/swine/QC/1792-1/2009 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI324057 A/swine/QC/1697-5/2009 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI324054 A/swine/QC/1697-4/2009 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI324051 A/swine/QC/1697-3/2009 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI324048 A/swine/QC/1697-2/2009 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI324043 A/swine/QC/1697-1/2009 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI324035 A/swine/QC/1695-3/2009 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI324029 A/swine/QC/1588/2009 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI324026 A/swine/QC/1533-5/2009 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI324023 A/swine/QC/1533-1/2009 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI324012 A/swine/QC/1531-2/2009 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI324009 A/swine/QC/1531-1/2009 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI324006 A/swine/QC/1488-5/2009 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI324003 A/swine/QC/1488-4/2009 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI324000 A/swine/QC/1488-3/2009 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI323995 A/swine/QC/1488-2/2009 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI323989 A/swine/QC/438-B/2009 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI323986 A/swine/QC/410-B/2009 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI323983 A/swine/QC/410-A/2009 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI323978 A/swine/QC/398/2009 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI323964 A/swine/QC/368/2009 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI323695 A/England/797/2009 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI323671 A/England/1116/2009 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI323434 A/England/213/2009 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI323166 A/swine/Texas/02512/2008 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI323119 A/swine/South Dakota/00731/2005 (A/H1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI323113 A/swine/Quebec/01759/2007 (A/H1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI323112 A/swine/Quebec/01758/2007 (A/H1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI322754 A/swine/North Carolina/00725/2005 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI322612 A/swine/Nebraska/01182/2006 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI322611 A/swine/Nebraska/01164/2006 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI322566 A/swine/Missouri/02012/2008 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI322564 A/swine/Missouri/01990/2008 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI322553 A/swine/Missouri/01461/2007 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI322331 A/swine/Minnesota/01835/2007 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI322329 A/swine/Minnesota/01831/2007 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI322328 A/swine/Minnesota/01813/2007 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI322279 A/swine/Minnesota/01415/2006 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI322228 A/swine/Minnesota/01120/2006 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI322172 A/swine/Minnesota/00575/2004 (A/H1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI322147 A/swine/Minnesota/00310/2004 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI322103 A/swine/Manitoba/01093/2006 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI322102 A/swine/Manitoba/01004/2006 (A/H1N2) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI321996 A/swine/Iowa/01895/2007 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI321975 A/swine/Iowa/00843/2005 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI321972 A/swine/Iowa/00807/2005 (A/H1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI321971 A/swine/Iowa/00748/2005 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI321458 A/FUKUI/67/2011 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI320737 A/Pavia/09/2011 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI320164 A/Vermont/04/2011 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI320147 A/Tennessee/02/2011 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI320136 A/Pennsylvania/08/2011 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI320129 A/Pennsylvania/07/2011 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI320121 A/Pennsylvania/02/2011 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI320104 A/New York/04/2011 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI320042 A/Massachusetts/09/2011 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI320039 A/Maryland/04/2011 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI319994 A/Arizona/02/2011 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI319989 A/Alaska/12/2011 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI319464 A/Estonia/55236/11 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI319447 A/Czech Republic/32/2011 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI319430 A/Algeria/G388/2010 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI316986 A/swine/Iowa/A0109004/2010 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI316985 A/swine/Iowa/A0109003/2010 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI316818 A/Mexico/InDRE2415/2011 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI316816 A/Mexico/InDRE2413/2011 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI316811 A/Mexico/InDRE2408/2011 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI316809 A/Mexico/InDRE2407/2011 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI316451 A/Texas/08/2011 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI316424 A/Pennsylvania/04/2011 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI316387 A/New Jersey/05/2011 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI315913 A/Mexico/InDRE2222/2011 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI315901 A/Mexico/InDRE2200/2011 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI315898 A/Mexico/InDRE2197/2011 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI315895 A/Mexico/InDRE2195/2011 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI315891 A/Mexico/InDRE2192/2011 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI315840 A/Mexico/InDRE1947/2011 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI315838 A/Mexico/InDRE1946/2011 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI315836 A/Mexico/InDRE1945/2011 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI312488 A/swine/Hong Kong/1152/2004 (A/H1N2) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI312486 A/swine/Hong Kong/NS788/2004 (A/H1N2) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI312484 A/swine/Hong Kong/NS777/2004 (A/H1N2) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI312482 A/swine/Hong Kong/NS776/2004 (A/H1N2) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI312480 A/swine/Hong Kong/1100/2004 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI311431 A/swine/Hong Kong/1111/2004 (A/H1N2) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI310173 A/Wisconsin/02/2011 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI310158 A/Virginia/04/2011 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI310136 A/Vermont/04/2011 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI310133 A/Utah/08/2011 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI310130 A/Texas/07/2011 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI310103 A/Pennsylvania/02/2011 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI310093 A/Oregon/03/2011 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI310075 A/North Carolina/22/2010 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI310069 A/North Carolina/03/2011 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI310055 A/New York/02/2011 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI310049 A/New Jersey/03/2011 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI310043 A/Nevada/05/2011 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI310040 A/Nevada/01/2011 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI310002 A/Massachusetts/06/2011 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI309999 A/Massachusetts/04/2011 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI309977 A/Maryland/05/2011 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI309970 A/Maryland/04/2011 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI309962 A/Maryland/03/2011 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI309946 A/Maine/03/2011 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI309943 A/Maine/01/2011 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI309892 A/Idaho/04/2011 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI309889 A/Georgia/25/2010 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI309803 A/Arizona/01/2011 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI308933 A/South Carolina/AF21803/2011 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI308930 A/Ohio/AF21800/2011 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI308926 A/North Carolina/AF21796/2011 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI308923 A/New Jersey/AF21791/2011 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI308913 A/Florida/AF21772/2011 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI308912 A/Florida/AF21771/2011 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI308911 A/District of Columbia/AF21769/2011 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI308910 A/Arizona/AF21768/2011 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI306130 A/Denmark/111/2010 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI304927 A/Galicia/RR6720/2010 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI304926 A/Galicia/RR6722/2010 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI304925 A/Galicia/RR6723/2010 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI304924 A/Galicia/RR6724/2010 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI304923 A/Galicia/RR6725/2010 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI304907 A/Galicia/RR6669/2010 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI304873 A/LaRioja/RR6478/2009 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI304729 A/CastillaLaMancha/RR4990/2009 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI302101 A/Georgia/21/2010 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI301814 A/Aragon/RR6925/2010 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI301719 A/Texas/14/2010 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI295399 A/El Salvador/798/2010 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI288290 A/Boston/672/2009 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI286988 A/CapeTown/29/2010 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI283773 A/Habana/7374/2010 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI282735 A/Mexico City/WRAIR1773T/2010 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI282135 A/Tallinn/INS374/2010 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI281919 A/Warsaw/INS316/2009 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI280320 A/Pennsylvania/07/2010 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI278896 A/Texas/03/2010 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI278878 A/Mexico/2880/2010 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI278127 A/Moldova/61/2010 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI278108 A/Moldova/30/2010 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI277857 A/Quito/WR1589N/2009 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI277541 A/Wurzburg/INS381/2009 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI276823 A/Japan/AF2287/2009 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI276812 A/Japan/AF2276/2009 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI276632 A/Germany/AF2210/2010 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI276629 A/Germany/AF2207/2010 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI276556 A/ENG/95100002/2009 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI276549 A/SC/98/2009 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI276521 A/ENG/94800108/2009 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI276459 A/SC/53/2009 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI276458 A/SC/51/2009 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI276394 A/ENG/745/2009 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI276363 A/ENG/740/2009 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI276348 A/SC/89/2009 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI273900 A/California/09/2010 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI273891 A/Dnipropetrovsk/445/2010 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI273838 A/Wisconsin/06/2010 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI273792 A/Wisconsin/03/2010 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI273744 A/Maryland/06/2010 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI273738 A/Minnesota/04/2010 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI273674 A/Arizona/06/2010 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI273662 A/Texas/03/2010 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI273656 A/Hawaii/06/2010 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI273626 A/Arizona/03/2010 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI272600 A/FUKUOKA-C/34/2010 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI272565 A/KAGOSHIMA/7/2010 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI272563 A/KAGOSHIMA/6/2010 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI272561 A/KAGOSHIMA/3/2010 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI272559 A/KAGOSHIMA/1/2010 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI272034 A/PHILIPPINES/824/2010 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI270655 A/California/VRDL4/2010 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI269967 A/Perth/500/2010 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI267816 A/Orenburg/IIV-13/2010 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI267664 A/California/VRDL6/2010 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI258782 A/Tallinn/INS183/2010 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI258726 A/Mexico City/014/2009 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI256534 A/Sichuan-Jiangyang/SWL1165/2009 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI256254 A/Beijing-Huairou/SWL1991/2009 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI255184 A/Haishu/SWL110/2010 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI254061 A/Orenburg/IIV-13/2010 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI253897 A/Sachsen/4/2010 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI247472 A/Vermont/30/2009 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI247317 A/Indiana/02/2010 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI247252 A/Colorado/03/2010 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI246174 A/Beijing/SE2649/2009 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI243994 A/Bosnia/1250/2009 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI240720 A/Milan/324/2009 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI240588 A/Eng/XFL00137/2009 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI240436 A/ENG/204/2009 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI234550 A/Russia/180/2009 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI234542 A/Russia/171/2009 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI234468 A/Catalonia/S2151/2009 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI231326 A/Mexico/2445/2009 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI230400 A/Russia/178/2009 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI230293 A/Salekhard/01/2009 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI228774 A/swine/Ontario/11112/04 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI227723 A/Minnesota/16/2009 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI226008 A/KOBE/91993/2009 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI226000 A/KOBE/91992/2009 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI221278 A/Catalonia/NS7362/2009 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI217414 A/Catalonia/S1687/2009 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI215844 A/SOUTH AUSTRALIA/2030/2009 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI186415 A/England/213/2009 (A/H1N1 swl) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI130577 A/swine/St-Hyacinthe/106/1991 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI72086 A/swine/Tennessee/23/1976 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI68609 A/swine/Minnesota/24/1975 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI63918 A/swine/Iowa/1/1977 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI62577 A/swine/Minnesota/27/1976 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI62048 A/swine/Tennessee/49/1977 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI62010 A/swine/Tennessee/21/1977 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)
EPI61991 A/swine/Tennessee/19/1977 (A/H1N1) segment 4 (HA) 27.0 6.903074e+00 14/14 (100%)

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