Rhiza Labs FluTracker Forum

Ontario Agency for Health Protection and Promotion has release an October, 2010 H1N1 sequence at Genbank, A/Ontario/130741/2010, which has S188T. It also has R208K and is related to A/North Carolina/14/2010. In addition, a November sequence, A/Ontario/3620/2010, has S186P.

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LOCUS CY081061 1777 bp cRNA linear VRL 01-FEB-2011
DEFINITION Influenza A virus (A/Ontario/130741/2010(H1N1)) hemagglutinin (HA)
gene, complete cds.
ACCESSION CY081061
VERSION CY081061.1 GI:321117558
DBLINK Project: 37813
KEYWORDS .
SOURCE Influenza A virus (A/Ontario/130741/2010(H1N1))
ORGANISM Influenza A virus (A/Ontario/130741/2010(H1N1))
Viruses; ssRNA negative-strand viruses; Orthomyxoviridae;
Influenzavirus A.
REFERENCE 1 (bases 1 to 1777)
AUTHORS Eshaghi,A., Alexander,D.C., Li,A., Ma,J.H., Low,D.E. and
Gubbay,J.B.
TITLE Characterization of influenza A (H1N1) virus strains from Ontario,
Canada
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 1777)
AUTHORS Eshaghi,A., Alexander,D.C., Li,A., Ma,J.H., Low,D.E. and
Gubbay,J.B.
TITLE Direct Submission
JOURNAL Submitted (31-JAN-2011) Public Health Laboratory - Toronto, Ontario
Agency for Health Protection and Promotion, 81 Resources Road,
Toronto, Ontario, Canada
FEATURES Location/Qualifiers
source 1..1777
/organism="Influenza A virus
(A/Ontario/130741/2010(H1N1))"
/mol_type="viral cRNA"
/strain="A/Ontario/130741/2010"
/serotype="H1N1"
/isolation_source="nasopharyngeal swab"
/host="human; gender M; age 50"
/db_xref="taxon:979622"
/segment="4"
/lab_host="GMKC1 passage(s)"
/country="Canada: Ontario"
/collection_date="13-Oct-2010"
gene 33..1733
/gene="HA"
CDS 33..1733
/gene="HA"
/function="receptor binding and fusion protein"
/codon_start=1
/product="hemagglutinin"
/protein_id="ADW54468.1"
/db_xref="GI:321117559"
/translation="MKAILVVLLYTFATANADTLCIGYHANNSTDTVDTVLEKNVTVT
HSVNLLEDKHNGKLCKLRGVAPLHLGKCNIAGWILGNPECESLSTASSWSYIVETSSS
DNGTCYPGDFIDYEELREQLSSVSSFERFEIFPKTSSWPNHDSNKGVTAACPHAGAKS
FYKNLIWLVKKGNSYPKLSKSYINDKGKEVLVLWGIHHPSTTADQQSLYQNADAYVFV
GTSKYSKKFKPEIAIRPKVRDQEGRMNYYWTLVEPGDKITFEATGNLVVPRYAFAMER
NAGSGIIISDTPVHDCNTTCQTPKGAINTSLPFQNIHPITIGKCPKYVKSTKLRLATG
LRNVPSIQSRGLFGAIAGFIEGGWTGMVDGWYGYHHQNEQGSGYAADLKSTQNAIDKI
TNKVNSVIEKMNTQFTAVGKEFNHLEKRIENLNKKVDDGFLDIWTYNAELLVLLENER
TLDYHDSNVKNLYEKVRNQLKNNAKEIGNGCFEFYHKCDNTCMESVKNGTYDYPKYSE
EAKLNREEIDGVKLESTRIYQILAIYSTVASSLVLVVSLGAISFWMCSNGSLQCRICI
"
sig_peptide 33..83
/gene="HA"
mat_peptide 84..1064
/gene="HA"
/product="HA1"
mat_peptide 1065..1730
/gene="HA"
/product="HA2"
ORIGIN
1 agcaaaagca ggggaaaaca aaagcaacaa aaatgaaggc aatactagta gttctgctat
61 atacatttgc aaccgcaaat gcagacacat tatgtatagg ttatcatgcg aacaattcaa
121 cagacactgt agacacagta ctagaaaaga atgtaacagt aacacactct gttaaccttc
181 tagaagacaa gcataacggg aaactatgca aactaagagg ggtagcccca ttgcatttgg
241 gtaaatgtaa cattgctggc tggatcctgg gaaatccaga gtgtgaatca ctctcaacag
301 caagctcatg gtcctacatt gtggaaacat ctagttcaga caatggaacg tgttacccag
361 gagatttcat cgattatgag gaactaagag agcaattgag ctcagtgtca tcatttgaaa
421 ggtttgagat attccccaag acaagttcat ggcccaatca tgactcgaac aaaggtgtaa
481 cggcagcatg tcctcatgct ggagcaaaaa gcttctacaa aaatttaata tggctagtta
541 aaaaaggaaa ttcataccca aagctcagca aatcctacat taatgataaa gggaaagaag
601 tcctcgtgct atggggcatt caccayccat ctactactgc tgaccaacaa agtctctatc
661 agaatgcaga tgcatatgtt tttgtgggga catcaaaata cagcaagaag ttcaagccgg
721 aaatagcaat aaggcccaaa gtgagggatc aagaagggag aatgaactat tactggacac
781 tagtagagcc gggagacaaa ataacattcg aagcaactgg aaatctagtg gtaccgagat
841 atgcattcgc aatggaaaga aatgctggat ctggtattat catttcagat acaccagtcc
901 acgattgcaa tacaacttgt cagacaccca agggtgctat aaacaccagc ctcccatttc
961 agaatataca tccgatcaca attggaaaat gtccaaaata tgtaaaaagc acaaaattga
1021 gactggccac aggattgagg aatgtcccgt ctattcaatc tagaggccta tttggggcca
1081 ttgccggctt cattgaaggg gggtggacag ggatggtaga tggatggtac ggttatcacc
1141 atcaaaatga gcaggggtca ggatatgcag ccgacctgaa gagcacacag aatgccattg
1201 acaagattac taacaaagta aattctgtta ttgaaaagat gaatacacag ttcacagcag
1261 taggtaaaga gttcaaccac ctggaaaaaa gaatagagaa tttaaataaa aaagttgatg
1321 atggtttcct ggacatttgg acttacaatg ccgaactgtt ggttctattg gaaaatgaaa
1381 gaactttgga ctaccacgat tcaaatgtga agaacttata tgaaaaggta agaaaccagt
1441 taaaaaacaa tgccaaggaa attggaaacg gctgctttga attttaccac aaatgcgata
1501 acacgtgcat ggaaagtgtc aaaaatggga cttatgacta cccaaaatac tcagaggaag
1561 caaaattaaa cagagaagaa atagatgggg taaagctgga atcaacaagg atttaccaga
1621 ttttggcgat ctattcaact gtcgccagtt cattggtact ggtagtctcc ctgggggcaa
1681 tcagtttctg gatgtgctct aatgggtctc tacagtgtag aatatgtatt taacattagg
1741 atttcagaag catgagaaaa acacccttgt ttctact

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www.twitter.com/hniman

LOCUS CY081069 1777 bp cRNA linear VRL 01-FEB-2011
DEFINITION Influenza A virus (A/Ontario/3620/2010(H1N1)) hemagglutinin (HA)
gene, complete cds.
ACCESSION CY081069
VERSION CY081069.1 GI:321117663
DBLINK Project: 37813
KEYWORDS .
SOURCE Influenza A virus (A/Ontario/3620/2010(H1N1))
ORGANISM Influenza A virus (A/Ontario/3620/2010(H1N1))
Viruses; ssRNA negative-strand viruses; Orthomyxoviridae;
Influenzavirus A.
REFERENCE 1 (bases 1 to 1777)
AUTHORS Eshaghi,A., Alexander,D.C., Li,A., Ma,J.H., Low,D.E. and
Gubbay,J.B.
TITLE Characterization of influenza A (H1N1) virus strains from Ontario,
Canada
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 1777)
AUTHORS Eshaghi,A., Alexander,D.C., Li,A., Ma,J.H., Low,D.E. and
Gubbay,J.B.
TITLE Direct Submission
JOURNAL Submitted (31-JAN-2011) Public Health Laboratory - Toronto, Ontario
Agency for Health Protection and Promotion, 81 Resources Road,
Toronto, Ontario, Canada
FEATURES Location/Qualifiers
source 1..1777
/organism="Influenza A virus (A/Ontario/3620/2010(H1N1))"
/mol_type="viral cRNA"
/strain="A/Ontario/3620/2010"
/serotype="H1N1"
/isolation_source="nasopharyngeal swab"
/host="human; gender F; age 53"
/db_xref="taxon:979621"
/segment="4"
/lab_host="GMKC1 passage(s)"
/country="Canada: Ontario"
/collection_date="24-Nov-2010"
gene 33..1733
/gene="HA"
CDS 33..1733
/gene="HA"
/function="receptor binding and fusion protein"
/codon_start=1
/product="hemagglutinin"
/protein_id="ADW54527.1"
/db_xref="GI:321117664"
/translation="MKAILVVLLYTFATASADTLCIGYHANNSTDTVDTVLEKNVTVT
HSVNLLEDKHNGKLCKLRGVAPLHLGKCNIAGWILGNPECESLSTASSWSYIVETSSS
DNGTCYPGDFIDYEELREQLSSVSSFERFEIFPKTSSWPNHDSNKGVTTACPHAGAKS
FYKNLIWLVKKGNSYPKLSKSYINDKGKEVLVLWGIHHPPTSADQQSLYQNADAYVFV
GTSRYSKKFKPEIAIRPKVRDQEGRMNYYWTLVEPGDKITFEATGNLVVPRYAFAMER
NAGSGIIISDTPVHDCNTTCQTPNGAINTSLPFQNIHPITIGKCPKYVKSTKLRLATG
LRNVPSIQSRGLFGAIAGFIEGGWTGMVDGWYGYHHQNEQGSGYAADLKSTQNAIDEI
TNKVNSVIEKMNTQFTAVGKEFNHLEKRIENLNKKVDDGFLDIWTYNAELLVLLENER
TLDYHDSNVKNLYEKVRSQLKNNAKEIGNGCFEFYHKCDNTCMESVKNGTYDYPKYSE
EAKLNREEIDGVKLESTRIYQILAIYSTVASSLVLVVSLGAISFWMCSNGSLQCRICI
"
sig_peptide 33..83
/gene="HA"
mat_peptide 84..1064
/gene="HA"
/product="HA1"
mat_peptide 1065..1730
/gene="HA"
/product="HA2"
ORIGIN
1 agcaaaagca ggggaaaaca aaagcaacaa aaatgaaggc aatactagta gttctgctat
61 atacatttgc aaccgcaagt gcagacacat tatgtatagg ttatcatgcg aacaattcaa
121 cagacactgt agacacagta ctagaaaaga atgtaacagt aacacactct gttaaccttc
181 tagaagacaa gcataacggg aaactatgca aactaagagg ggtagcccca ttgcatttgg
241 gtaaatgtaa cattgctggc tggatcctgg gaaatccaga gtgtgaatca ctctccacag
301 caagctcatg gtcctacatt gtggaaacat ctagttcaga caatggaacg tgttacccag
361 gagatttcat cgattatgag gagctaagag agcaattgag ctcagtgtca tcatttgaaa
421 ggtttgagat attccccaag acaagttcat ggcccaatca tgactcgaac aaaggtgtaa
481 cgacagcatg tcctcatgct ggagcaaaaa gcttctacaa aaacttaata tggctagtta
541 aaaaaggaaa ttcataccca aaactcagca aatcctacat taatgacaaa gggaaagaag
601 tcctcgtgct atggggcatt caccatccac ctactagtgc tgaccaacaa agtctctatc
661 agaatgcaga tgcatatgtt tttgtgggga catcaagata cagcaagaag ttcaagccgg
721 aaatagcaat aagacccaaa gtgagggatc aagaagggag gatgaactat tactggacac
781 tagtagagcc gggagacaaa ataacattcg aagcaactgg aaatctagtg gtaccgagat
841 atgcattcgc aatggaaaga aatgctggat ctggtattat catttcagat acaccagtcc
901 acgattgcaa tacaacttgt cagacaccca atggtgctat aaacaccagc ctcccatttc
961 agaatataca tccgatcaca attggaaaat gtccaaaata tgtaaaaagc acaaaattga
1021 gactggccac aggattgagg aatgtcccgt ctattcaatc tagaggccta tttggggcca
1081 ttgccggttt cattgaaggg gggtggacag ggatggtaga tggatggtac ggttatcacc
1141 atcaaaatga gcaggggtca gggtatgcag ccgacctgaa gagcacacag aatgccattg
1201 acgagattac taacaaagta aattctgtta ttgaaaagat gaatacacag ttcacagcag
1261 taggtaaaga gttcaaccac ctggaaaaaa gaatagagaa tttaaataaa aaagttgatg
1321 atggtttcct ggacatttgg acttacaatg ccgaactgtt ggttctattg gaaaatgaaa
1381 gaactttgga ctaccacgat tcaaatgtga agaacttata tgaaaaggta agaagccagt
1441 taaaaaacaa tgccaaggaa attggaaacg gctgctttga attttaccac aaatgtgata
1501 acacgtgcat ggaaagtgtc aaaaatggga cttatgacta cccaaaatac tcagaggaag
1561 caaaattaaa cagagaagaa atagatgggg taaaactgga atcaacaagg atttaccaga
1621 ttttggcgat ctattcaact gtcgccagtt cattggtact ggtagtctcc ctgggggcaa
1681 tcagtttctg gatgtgctct aatgggtctc tacagtgtag aatatgtatt taacattagg
1741 atttcagaag catgagaaaa acacccttgt ttctact

_________________
www.twitter.com/hniman

S188T in A/Ontario/130741/2010 and A/Ontario/720545/2010.

_________________
www.twitter.com/hniman

S186P in A/Ontario/804635/2010, A/Ontario/778873/2010, A/Ontario/741328/2010, A/Ontario/3620/2010.

_________________
www.twitter.com/hniman

Of the six HA sequences released from Ontario, 4 had S186P and 2 had S188T (sequences for all 8 gene segments were released).

_________________
www.twitter.com/hniman

HA from Ontario/778873 is identical to England/125. Ontario/804635 is VERY close. Ontario/741328 and Ontario/3620 are identical with each other and close to England/125.

_________________
www.twitter.com/hniman