Rhiza Labs FluTracker Forum

Harbin is China's national lab. The does a lot of sequencing. The "probable errors" are just more fantasy on your part. There is a lot of recombination in northern China (as seen in published sequences). In southern China, the H5N1 recombination is covered up with partial and withheld sequences.

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The sequences with mixtures (pseudospecies) continue to increase. Today's sequences from Korea have H274Y as a mixture, which was also true for the Hong Kong ex-San Francisco fit sequences. Similarly, the father in Italy had G158E and D225G and a rare polymorphism. He infected his son, who had the rare polymorphism and D225G, but not G158E because G158E was a pseudospecies (in Italy as well as many examples in US).

So now the key players, D225G, G158E, and H274Y are being reported as pseudospecies, and they are also showing up together, like the father with D225G and G158E, or the patient in Korea with G158E and H274Y, or the Duke death cluster with D225G and H274Y.

H1N1 knows what it is doing, no random mutations or traveling salesmen required.

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so Harbin was wrong twice with this one sequence, they had to correct it twice.
No reason was given.
The first correction even had 23 changes plus one deletion and was only done after
3 years.


what was with the retracted Chinese swine sequences (monotreme)
also Harbin, the Canada/1963 sequences, the Vietnam sequences
from eggshells almost unmutated for 8 years,...

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no patents on genes, publish the GISAID sequences !

when you have a mixture, then why replace the old sequence ?
just publish both. Especially in such an important case with a deletion.

That had been done with other sequences,
although rarely.

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no patents on genes, publish the GISAID sequences !

They are both published. In fact all three are published. Same thing for the Shnaghai pH1N1 sequences with E627K. One sequence replaced the other, but they can both be accessed. Most labs just hold the sequences they don't like, or publish partials and withhold the part of the sequence they don't like, especially H5N1 sequences in Asia, where the recombination is VERY common, withheld sequences notwithstanding.

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or there was an error.
Contamination, wrong sample, data/computer-error

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no patents on genes, publish the GISAID sequences !

No, the recombination is withheld. One sequence has recombination, while subsequent sequences start with the same sequence and stop as soon as the recombination point is reached.
Almost all 2001/2002 H5N1 sequences from Hong Kong were partials, but even withholding half of the gene couldn't hide MANY examples of recombination in the sequences the withhold lab released.

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Although the 3' half of the S16 PB2 gene was missing from GenBank, the 5' half of NP was present, and it also contained recombination between Korean and Hong Kong avian genes. The recombinant contains sequences that differ from A/guineafowl/Hong Kong/NT184/03 by only 1 bp in the first 234 bp deposited at GenBank. The remaining deposited sequences then matched other 2003 H9N2 Korean isolates. This region in S4 was not deposited at GenBank so it was not possible to determine if a similar recombination had happened in S4.

http://www.recombinomics.com/News/03050 ... ation.html

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if the recombination is real, then why should they hide it ?

If OTOH it is created in the laboratory due to contamination, then they
would have an interest to hide it.

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no patents on genes, publish the GISAID sequences !

Please. These labs have been denying recombination for years. Their entire carreers are based on no recombination.

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