Rhiza Labs FluTracker Forum

The place to discuss the flu
It is currently Mon May 20, 2013 1:10 pm

All times are UTC - 5 hours [ DST ]




Post new topic Reply to topic  [ 111 posts ]  Go to page Previous  1 ... 3, 4, 5, 6, 7, 8, 9 ... 12  Next
Author Message
PostPosted: Tue Mar 16, 2010 11:54 pm 
Offline
User avatar

Joined: Tue Aug 11, 2009 2:58 pm
Posts: 1400
Location: Katy, TX
littlebird wrote:
sutter58 wrote:
I say we have gone this route before with the Ukranian situation. With Influenza it is always changing, so I think the change is worth monitoring, but I am with the others in the article. Keep and eye on it. What else can be done. Nothing.
The h1n1 started way before last April. Many have said it stared late 2008. The word is get vaccinated.



[url=
I don't know sutter, something tells me we're not in Kansas anymore...



Please don't mention Kansas and pandemic swine flu together. :)

_________________
I am not a doctor, virologist, or any of type of medical/life sciences professional.

I am a layman with a background in the physical sciences.


Top
 Profile  
 
PostPosted: Tue Mar 16, 2010 11:58 pm 
Offline

Joined: Sat Dec 12, 2009 8:43 pm
Posts: 469
Thank you.

So that implies that virulence will increase, probably resulting in higher viral loads, and the associated change in mortality will then depend more or less on whether that fixing promotes deeper lung penetration via a mixture with D225G? So if no association occurs, the virus may become milder. But if an association occurs, pretty much all hell could conceivably break loose?

I believe you mentioned previously that D225G might become a prominent player. If that becomes true, and a mixture occurs with E627K, I would imagine that would set the stage for a near worst-case scenario. Am I way off the mark?


Top
 Profile  
 
PostPosted: Wed Mar 17, 2010 12:00 am 
Online

Joined: Wed Aug 19, 2009 10:42 am
Posts: 27318
Location: Pittsburgh, PA USA
Pandora wrote:
Thank you.

So that implies that virulence will increase, probably resulting in higher viral loads, and the associated change in mortality will then depend more or less on whether that fixing promotes deeper lung penetration via a mixture with D225G? So if no association occurs, the virus may become milder. But if an association occurs, pretty much all hell could conceivably break loose?

I believe you mentioned previously that D225G might become a prominent player. If that becomes true, and a mixture occurs with E627K, I would imagine that would set the stage for a near worst-case scenario. Am I way off the mark?

They were together in 1918.

_________________
www.twitter.com/hniman


Top
 Profile  
 
PostPosted: Wed Mar 17, 2010 12:17 am 
Offline

Joined: Sat Dec 12, 2009 8:43 pm
Posts: 469
Lovely...


Top
 Profile  
 
PostPosted: Wed Mar 17, 2010 3:30 am 
Offline

Joined: Sat Jan 30, 2010 2:44 am
Posts: 887
niman wrote:
E627K in pandemic H1N1 sets the stage for significant changes.


What sort of significant changes?


Top
 Profile  
 
PostPosted: Wed Mar 17, 2010 4:06 am 
Online
User avatar

Joined: Tue Sep 01, 2009 11:54 pm
Posts: 1775
Location: germany
can someone access
http://mbio.asm.org/mbio00067-10v1.pdf

I only get an empty page

_________________
no patents on genes, publish the GISAID sequences !


Top
 Profile  
 
PostPosted: Wed Mar 17, 2010 5:26 am 
Offline

Joined: Fri Jan 08, 2010 3:22 pm
Posts: 5180
Location: East of London
Quote:
I only get an empty page


The file is large (12MB) and about 25 pages long. It takes a while to load, just be patient. Looks interesting and will take a peak later.

The PB2 E627K Mutation Attenuates Viruses Containing the 2009
H1N1 Influenza Pandemic Polymerase

_________________
Praemonitus, Praemunitus..Forewarned is Forearmed.


Top
 Profile  
 
PostPosted: Wed Mar 17, 2010 6:09 am 
Online

Joined: Wed Aug 19, 2009 10:42 am
Posts: 27318
Location: Pittsburgh, PA USA
stephensons wrote:
Quote:
I only get an empty page


The file is large (12MB) and about 25 pages long. It takes a while to load, just be patient. Looks interesting and will take a peak later.

The PB2 E627K Mutation Attenuates Viruses Containing the 2009
H1N1 Influenza Pandemic Polymerase

Changing PB2 to E627K did not increase replication of CA/04/09, but changing the 1918 virus from E627K to E627 reduced virus.

_________________
www.twitter.com/hniman


Top
 Profile  
 
PostPosted: Wed Mar 17, 2010 8:12 am 
Online

Joined: Wed Aug 19, 2009 10:42 am
Posts: 27318
Location: Pittsburgh, PA USA
stephensons wrote:
Quote:
I only get an empty page


The file is large (12MB) and about 25 pages long. It takes a while to load, just be patient. Looks interesting and will take a peak later.

The PB2 E627K Mutation Attenuates Viruses Containing the 2009
H1N1 Influenza Pandemic Polymerase

The paper is an artificial system that says little about pandemic H1N1. The finding that E627K reduces levels of the rescued reassortant, even though E627K is found in the patients in India indicates the experimental results are not applicable to pandemic H1N1.

Here is the key quote from the paper:

We show that in both A549 cell culture and in a mouse model of influenza infection, two of these
changes, PB2-E627K and –D701N, do not potentiate the replication or virulence of a virus
containing the 2009 pandemic RNP. Rather, both of these changes significantly impair the
replication of the virus and diminish the histopathological consequences of infection. Our
findings suggest that if the 2009 pandemic virus were to acquire these changes, greater virulence
would be an unlikely consequence.

_________________
www.twitter.com/hniman


Last edited by niman on Wed Mar 17, 2010 8:38 am, edited 1 time in total.

Top
 Profile  
 
PostPosted: Wed Mar 17, 2010 8:26 am 
Offline

Joined: Sat Jan 30, 2010 2:44 am
Posts: 887
On a related note re India:

From what I have read, there are more similarities with the current pandemic swine flu and Spanish Flu, than either of the two later pandemics.

From memory, a nasty wave in India occurred at the same time as one in the USA during the Spanish Flu. India could be useful to watch.


Top
 Profile  
 
Display posts from previous:  Sort by  
Post new topic Reply to topic  [ 111 posts ]  Go to page Previous  1 ... 3, 4, 5, 6, 7, 8, 9 ... 12  Next

All times are UTC - 5 hours [ DST ]


Who is online

Users browsing this forum: meteorjosh, niman, stevevit and 126 guests


You cannot post new topics in this forum
You cannot reply to topics in this forum
You cannot edit your posts in this forum
You cannot delete your posts in this forum
You cannot post attachments in this forum

Search for:
Jump to:  
Powered by phpBB® Forum Software © phpBB Group