Rhiza Labs FluTracker Forum

NIID just released two HA sequences at GISAID. One, A/YAMAGUCHI/247/2009, had A716G, which codes for D225G, but it also has T717A, which codes for D225E, as well as additional markers fro D225E. Thus, D225G has been appended to a D225E genetic background.

C940T travel log

gb|CY054979.1| Influenza A virus (A/California/VRDL55/2009(H1... 32.2 12
gb|CY054939.1| Influenza A virus (A/California/VRDL48/2009(H1... 32.2 12
gb|CY054931.1| Influenza A virus (A/California/VRDL45/2009(H1... 32.2 12
gb|CY054899.1| Influenza A virus (A/California/VRDL39/2009(H1... 32.2 12
gb|CY054891.1| Influenza A virus (A/California/VRDL38/2009(H1... 32.2 12
gb|CY054851.1| Influenza A virus (A/California/VRDL32/2009(H1... 32.2 12
gb|CY054715.1| Influenza A virus (A/California/VRDL5/2009(H1N... 32.2 12
gb|GU369672.1| Influenza A virus (A/Ankara/28/2009(H1N1)) seg... 32.2 12
gb|CY052951.1| Influenza A virus (A/Texas/45061755/2009(H1N1)... 32.2 12
gb|CY052631.1| Influenza A virus (A/Texas/45061670/2009(H1N1)... 32.2 12
gb|CY052607.1| Influenza A virus (A/Texas/45034157/2009(H1N1)... 32.2 12
dbj|AB535745.1| Influenza A virus (A/Nagasaki/HA-53/2009(H1N1... 32.2 12
dbj|AB535744.1| Influenza A virus (A/Nagasaki/HA-52/2009(H1N1... 32.2 12
dbj|AB530488.1| Influenza A virus (A/Nagasaki/HA-44/2009(H1N1... 32.2 12
gb|GQ457514.1| Influenza A virus (A/California/25/2009(H1N1))... 32.2 12
gb|GQ422381.1| Influenza A virus (A/Milan/83/2009(H1N1)) segm... 32.2 12
gb|GQ387430.1| Influenza A virus (A/Catalonia/378/2009(H1N1))... 32.2 12
gb|GQ387382.1| Influenza A virus (A/Italy/172/2009(H1N1)) hem... 32.2 12
gb|GQ351314.1| Influenza A virus (A/Hong Kong/2369/2009(H1N1)... 32.2 12

Acquisition of D225G on a D225E background has been described previously

http://www.recombinomics.com/News/12020 ... weden.html

Note that each of the above sequences, which like Yokahama/247 has C940T, has D225E, including the Hong Kong isolate with H274Y.

However, the Yokahama isolates has appended D716A onto the D225E background and converted D225E to D225G. This D225E background is distinct from the D225E in Europe, which also has examples of D225G on a D225E background.

TN17, which has H274Y and D225E, also has C940T.

A/Shiga/43/2009 also has C940T as well as D225E and H274Y. the clustering of H274Y in this sub-clade provides addition evidnece for the transmission of H274Y in the US and Japan.

Commentary

http://www.recombinomics.com/News/01291 ... Japan.html