Rhiza Labs FluTracker Forum
http://fluboard.rhizalabs.com/forum/

CFIA Withholds H5N2 Sequencing Data For Fraser Valley
http://fluboard.rhizalabs.com/forum/viewtopic.php?f=5&t=13074
Page 1 of 2

Author:  niman [ Tue Dec 09, 2014 3:52 pm ]
Post subject:  CFIA Withholds H5N2 Sequencing Data For Fraser Valley

The OIE update from the CFIA acknowledges the presence of a polybasic cleavage site and partial sequencing, but has withheld the sequence of the polybasic cleavage site and the lineage.

Author:  niman [ Tue Dec 09, 2014 3:56 pm ]
Post subject:  Re: CFIA Withholds H5N2 Sequencing Data For Fraser Valley

Information received on 09/12/2014 from Dr Martine Dubuc, OIE Delegate for Canada, Chief Food Safety Officer Vice-President, Science Branch, Health Ministry, Canadian Food Inspection Agency, Ottawa, Canada
Summary
Report type Follow-up report No. 1
Date of start of the event 30/11/2014
Date of pre-confirmation of the event 01/12/2014
Report date 09/12/2014
Date submitted to OIE 09/12/2014
Reason for notification Reoccurrence of a listed disease
Date of previous occurrence 01/2008
Manifestation of disease Clinical disease
Causal agent Highly pathogenic avian influenza virus
Serotype H5N2
Nature of diagnosis Laboratory (advanced)
This event pertains to a defined zone within the country
Related reports Immediate notification (03/12/2014)
Follow-up report No. 1 (09/12/2014)
New outbreaks (3)
Outbreak 1 (BC-2014-NAI-003) Abbotsford, BRITISH COLUMBIA
Date of start of the outbreak 02/12/2014
Outbreak status Continuing (or date resolved not provided)
Epidemiological unit Farm
Affected animals
Species Susceptible Cases Deaths Destroyed Slaughtered
Birds 14000 250 250 13750 0
Affected population BC-2014-NAI-003 Chicken broiler breeder farm. Affected barn holds hens and roosters 37 week old (sudden death). In addition, 2 week old pullets on the premises with normal mortality rate.
Outbreak 2 (BC-2014-NAI-004) Abbotsford, BRITISH COLUMBIA
Date of start of the outbreak 02/12/2014
Outbreak status Continuing (or date resolved not provided)
Epidemiological unit Farm
Affected animals
Species Susceptible Cases Deaths Destroyed Slaughtered
Birds 27000 22 22 26978 0
Affected population BC-2014-NAI-004 Chicken broiler breeder farm. Affected barn holds hens and roosters 41 week old (sudden death). In addition, 7 and 59 week old hens and roosters on the premises with normal mortality rates.
Outbreak 3 (BC-2014-NAI-005) Abbotsford, BRITISH COLUMBIA
Date of start of the outbreak 04/12/2014
Outbreak status Continuing (or date resolved not provided)
Epidemiological unit Farm
Affected animals
Species Susceptible Cases Deaths Destroyed Slaughtered
Birds 31000 50 50
Affected population BC-2014-NAI-005 Meat turkey farm. One barn affected 9 week old birds, high mortality. In addition, 4 and 14 week old birds on premises with normal mortality rates.
Summary of outbreaks Total outbreaks: 3
Total animals affected
Species Susceptible Cases Deaths Destroyed Slaughtered
Birds 72000 322 322 40728 0
Outbreak statistics
Species Apparent morbidity rate Apparent mortality rate Apparent case fatality rate Proportion susceptible animals lost*
Birds 0.45% 0.45% 100.00% **
*Removed from the susceptible population through death, destruction and/or slaughter
**Not calculated because of missing information
Epidemiology
Source of the outbreak(s) or origin of infection
Unknown or inconclusive
Epidemiological comments Testing at the Canadian Food Inspection Agency (CFIA) National Centre for Foreign Animal Disease (NCFAD) on samples from outbreaks number BC-2014-NAI-001 and BC-2014-NAI-002 was performed: RT-PCR and partial sequencing of the H and N gene segments indicates a poly-basic cleavage site in H5 consistent with Highly Pathogenic Avian Influenza Virus and the virus being a H5N2 Avian Influenza Virus with an IVPI of 2.96-2.98. The CFIA has also confirmed H5 avian influenza virus in 3 additional farms (outbreak number BC-2014-NAI-003, BC-2014-NAI-004 and BC-2014-NAI-005) with clinical signs. Farms from outbreak number BC-2014-NAI-001, BC-2014-NAI-002, BC-2014-NAI-003 and BC-2014-NAI-004 have been humanely depopulated. At this point, this event is limited geographically to a portion of the Fraser Valley in the southern fringe of the province of British Columbia. Infected premises were quarantined by the Canadian Food Inspection Agency (CFIA) and a stamping out policy is being implemented, as described in the Notifiable Avian Influenza (NAI) Hazard Specific Plan. The Canadian Notifiable Avian Influenza Surveillance System (CanNAISS) is an ongoing surveillance system built to demonstrate absence of NAI in poultry in Canada. CanNAISS results and epidemiological investigation to date are supporting that there is no evidence of infection outside of the Fraser Valley in British Columbia. On 8 December 2014, the Canadian Food Inspection Agency (CFIA) has established a primary control zone in the area where the disease has been identified. The primary control zone is bordered on the west by the Pacific Ocean, on the south by the United States border, on the north by Highway 16, and on the east by the border between British Columbia and Alberta. Additional information on the zone and the restrictions in place are available at http://www.inspection.gc.ca/an.imals/te ... 8073267337.
Control measures
Measures applied
Stamping out
Quarantine
Movement control inside the country
Screening
Zoning
Vaccination prohibited
No treatment of affected animals
Measures to be applied
Disinfection of infected premises/establishment(s)
Diagnostic test results
Laboratory name and type Species Test Test date Result
National Centre for Foreign Animal Disease, Canadian Food Inspection Agency (National laboratory) Birds intravenous pathogenicity index (IVPI) test 06/12/2014 Positive
National Centre for Foreign Animal Disease, Canadian Food Inspection Agency (National laboratory) Birds real-time reverse transcriptase/polymerase chain reaction (RRT-PCR) 03/12/2014 Positive
National Centre for Foreign Animal Disease, Canadian Food Inspection Agency (National laboratory) Birds virus isolation 04/12/2014 Positive
National Centre for Foreign Animal Disease, Canadian Food Inspection Agency (National laboratory) Birds virus sequencing 04/12/2014 Positive
Future Reporting
The event is continuing. Weekly follow-up reports will be submitted.

http://www.oie.int/wahis_2/public/wahid ... rtid=16700

Author:  niman [ Tue Dec 09, 2014 4:13 pm ]
Post subject:  Re: CFIA Withholds H5N2 Sequencing Data For Fraser Valley

Testing at the Canadian Food Inspection Agency (CFIA) National Centre for Foreign Animal Disease (NCFAD) on samples from outbreaks number BC-2014-NAI-001 and BC-2014-NAI-002 was performed: RT-PCR and partial sequencing of the H and N gene segments indicates a poly-basic cleavage site in H5 consistent with Highly Pathogenic Avian Influenza Virus and the virus being a H5N2 Avian Influenza Virus with an IVPI of 2.96-2.98. The CFIA has also confirmed H5 avian influenza virus in 3 additional farms (outbreak number BC-2014-NAI-003, BC-2014-NAI-004 and BC-2014-NAI-005) with clinical signs. Farms from outbreak number BC-2014-NAI-001, BC-2014-NAI-002, BC-2014-NAI-003 and BC-2014-NAI-004 have been humanely depopulated. At this point, this event is limited geographically to a portion of the Fraser Valley in the southern fringe of the province of British Columbia. Infected premises were quarantined by the Canadian Food Inspection Agency (CFIA) and a stamping out policy is being implemented, as described in the Notifiable Avian Influenza (NAI) Hazard Specific Plan. The Canadian Notifiable Avian Influenza Surveillance System (CanNAISS) is an ongoing surveillance system built to demonstrate absence of NAI in poultry in Canada. CanNAISS results and epidemiological investigation to date are supporting that there is no evidence of infection outside of the Fraser Valley in British Columbia. On 8 December 2014, the Canadian Food Inspection Agency (CFIA) has established a primary control zone in the area where the disease has been identified. The primary control zone is bordered on the west by the Pacific Ocean, on the south by the United States border, on the north by Highway 16, and on the east by the border between British Columbia and Alberta. Additional information on the zone and the restrictions in place are available at http://www.inspection.gc.ca/an.imals/te ... 8073267337.

Author:  niman [ Tue Dec 09, 2014 4:41 pm ]
Post subject:  Re: CFIA Withholds H5N2 Sequencing Data For Fraser Valley

niman wrote:
RT-PCR and partial sequencing of the H and N gene segments indicates a poly-basic cleavage site in H5 consistent with Highly Pathogenic Avian Influenza Virus and the virus being a H5N2 Avian Influenza Virus with an IVPI of 2.96-2.98.

European Union legislation on avian influenza is contained in Council Directive
92/40/EEC. The disease is defined as follows in Annex III of the directive;
“For the purpose of the diagnostic procedures for the confirmation and differential
diagnosis of avian influenza the following definition shall apply.
‘Avian influenza’ means an infection of poultry caused by any influenza A virus which
has an intravenous pathogenicity index1 in six-week-old chickens greater than 1.2 or
any infection with influenza A viruses of H5 or H7 subtype for which nucleotide
sequencing has demonstrated the presence of multiple basic amino acids at the
cleavage site of the haemagglutinin.”
http://ec.europa.eu/food/fs/sc/scah/out45-final_en.pdf

Author:  niman [ Tue Dec 09, 2014 4:52 pm ]
Post subject:  Re: CFIA Withholds H5N2 Sequencing Data For Fraser Valley

The premises where the disease was diagnosed is in the Fraser Valley in the province of British Columbia, where an important event of highly pathogenic avian influenza also occurred in 2004. British Columbia is located at the westernmost limit of Canada. Birds from this farm were submitted by the veterinary private practitioner to the Animal Health Centre (AHC - British Columbia Ministry of Agriculture, Food and Fisheries) as a routine submission for a respiratory related problem with no significant mortality. The AHC is a member of the Canadian Avian Influenza Laboratory Network. The Canadian Food Inspection Agency (CFIA) National Center for Foreign Animal Diseases confirmed the diagnosis (conventional PCR H5 positive) on samples initially processed by the AHC. Results of gene sequencing indicate a low path cleavage site (NVPQRETR/GLFGAIA). The sequence is 99% related to another H5 virus (H5N2) from California in 2007. Closest match in Genbank is A/American green-winged teal/California/HKWF609/2007(H5N2), accession number: CY033444 Additional tests are underway. The index premises and all premises within 3 km of this premises have been placed under quarantine. Trace out investigations are underway and all significant contacts will also be quarantined. Surveillance and control activities have started in accordance with the CFIA Notifiable Avian Influenza Hazard Specific Plan. Note by the OIE Animal Health Information Department: H5 and H7 avian influenza in its low pathogenic form in poultry is a notifiable disease as per Chapter 10.4. on avian influenza of the Terrestrial Animal Health Code (2008).

http://www.oie.int/wahis_2/public/wahid ... ortid=7720

Author:  niman [ Tue Dec 09, 2014 5:37 pm ]
Post subject:  Re: CFIA Withholds H5N2 Sequencing Data For Fraser Valley

The Government of Canada is making more data and information available to Canadians than ever before. Canadians are also being offered more opportunities to participate in discussions on government policies and priorities

As a regulator, Health Canada plays an important role in protecting the health and safety of Canadians. Greater transparency and openness with Canadians strengthens the trust in our regulatory decisions.

The Regulatory Transparency and Openness Framework for Health Canada supports our commitment to the Government's overall Next link will take you to another Web site Open Government initiative. This will ultimately support Canadians in making better decisions about their health.

http://www.hc-sc.gc.ca/home-accueil/rto ... ex-eng.php

Author:  niman [ Tue Dec 09, 2014 7:13 pm ]
Post subject:  Re: CFIA Withholds H5N2 Sequencing Data For Fraser Valley

Commentary

http://www.recombinomics.com/News/12091 ... hheld.html

Author:  Jon Schultz [ Tue Dec 09, 2014 7:48 pm ]
Post subject:  Re: CFIA Withholds H5N2 Sequencing Data For Fraser Valley

niman wrote:


Thank you, Dr. Niman.

Author:  niman [ Tue Dec 09, 2014 8:00 pm ]
Post subject:  Re: CFIA Withholds H5N2 Sequencing Data For Fraser Valley

Jon Schultz wrote:
niman wrote:


Thank you, Dr. Niman.

Thank CFIA - Their actions leave little doubt that Fujian is now in North America (and probably US).

Author:  niman [ Wed Dec 10, 2014 6:47 am ]
Post subject:  Re: CFIA Withholds H5N2 Sequencing Data For Fraser Valley

Follow-up report No.1
Report reference: CAN-2014-NAI-001., Reference OIE : 16700, Report Date : 09/12/2014, Country : Canada
Report Summary
Name of sender of the report Dr Martine Dubuc Telephone +1 613 773 5722
Position OIE Delegate for Canada, Chief Food Safety Officer Vice-President,
Science Branch
Fax +1 613 773 5797
Address Floor 3, Room 349
1400 Merivale Road, Tower 2
Ottawa, Ontario K1A 0Y9 Ottawa
Email martine.dubuc@inspection.gc.ca
Date submitted to OIE 09/12/2014
Animal type Terrestrial Date of report 09/12/2014
Disease Highly pathogenic avian influenza Date of start of the event 30/11/2014
Causal Agent Highly pathogenic avian influenza virus Date of pre-confirmation of the
event
01/12/2014
Serotype(s) H5N2 Date of last occurrence 01/2008
Reason Reoccurrence of a listed disease Diagnosis Laboratory (advanced)
Country or zone a zone or compartment Clinical signs Yes
Number of reported outbreaks submitted= 5, Draft= 0
Outbreak details
Province Number of outbreaks District Sub-district Unit Type Location Latitude Longitude Start Date End Date:
BRITISH
COLUMBIA-other report
- submitted
- Farm Abbotsford 49.052273 -122.30595 30/11/2014
Species Measuring units Susceptible Cases Deaths Destroyed Slaughtered
Birds Animals 28000 7700 7700 20300 0
Affected Population BC-2014-NAI-002
Meat turkey farm
Affected barn: 83 days of age toms (70% mortality).
In addition, hens and 2 week old poults on the premises with normal mortality rate.
Province Number of outbreaks District Sub-district Unit Type Location Latitude Longitude Start Date End Date:
BRITISH
COLUMBIA-other report
- submitted
- Farm Chilliwack 49.171042 -121.95099 30/11/2014
Species Measuring units Susceptible Cases Deaths Destroyed Slaughtered
Birds Animals 12800 700 700 12100 0
Affected Population BC-2014-NAI-001
Chicken broiler breeder farm.
The affected barn holds a flock of 24 weeks of age (10% mortality).
An adjacent barn, not affected, contains a flock of 47 weeks of age.
Province Number of outbreaks District Sub-district Unit Type Location Latitude Longitude Start Date End Date:
BRITISH COLUMBIA-
(this report - submitted)
- Farm Abbotsford 49.052273 -122.30595 02/12/2014
Species Measuring units Susceptible Cases Deaths Destroyed Slaughtered
Birds Animals 14000 250 250 13750 0
Affected Population BC-2014-NAI-003
Chicken broiler breeder farm.
Affected barn holds hens and roosters 37 week old (sudden death).
In addition, 2 week old pullets on the premises with normal mortality rate.
Printed on: Tue Dec 9 19:41:45 CET 2014 Page 1/4Species Measuring units Susceptible Cases Deaths Destroyed Slaughtered
Province Number of outbreaks District Sub-district Unit Type Location Latitude Longitude Start Date End Date:
BRITISH COLUMBIA-
(this report - submitted)
- Farm Abbotsford 49.052273 -122.30595 02/12/2014
Species Measuring units Susceptible Cases Deaths Destroyed Slaughtered
Birds Animals 27000 22 22 26978 0
Affected Population BC-2014-NAI-004
Chicken broiler breeder farm.
Affected barn holds hens and roosters 41 week old (sudden death).
In addition, 7 and 59 week old hens and roosters on the premises with normal mortality rates.
Province Number of outbreaks District Sub-district Unit Type Location Latitude Longitude Start Date End Date:
BRITISH COLUMBIA-
(this report - submitted)
- Farm Abbotsford 49.052275 -122.30597 04/12/2014
Species Measuring units Susceptible Cases Deaths Destroyed Slaughtered
Birds Animals 31000 50 50 ... ...
Affected Population BC-2014-NAI-005
Meat turkey farm.
One barn affected 9 week old birds, high mortality. In addition, 4 and 14 week old birds on premises with normal mortality rates.
Outbreak summary: Total outbreaks = 5 (Submitted)
Species Susceptible Cases Deaths Destroyed Slaughtered
Birds 112800 8722 8722 73128 0
Epidemiology
Epidemiological comments
Testing at the Canadian Food Inspection Agency (CFIA) National Centre for Foreign Animal Disease (NCFAD) on samples from outbreaks number BC-2014-NAI-001 and BC-2014-NAI-002 was performed: RT-PCR and partial sequencing of the H and N gene segments indicates a
poly-basic cleavage site in H5 consistent with Highly Pathogenic Avian Influenza Virus and the virus being a H5N2 Avian Influenza Virus with an IVPI of 2.96-2.98.
The CFIA has also confirmed H5 avian influenza virus in 3 additional farms (outbreak number BC-2014-NAI-003, BC-2014-NAI-004 and BC-2014-NAI-005) with clinical signs.
Farms from outbreak number BC-2014-NAI-001, BC-2014-NAI-002, BC-2014-NAI-003 and BC-2014-NAI-004 have been humanely depopulated.
At this point, this event is limited geographically to a portion of the Fraser Valley in the southern fringe of the province of British Columbia. Infected premises were quarantined by the Canadian Food Inspection Agency (CFIA) and a stamping out policy is being implemented, as
described in the Notifiable Avian Influenza (NAI) Hazard Specific Plan. The Canadian Notifiable Avian Influenza Surveillance System (CanNAISS) is an ongoing surveillance system built to demonstrate absence of NAI in poultry in Canada. CanNAISS results and epidemiological
investigation to date are supporting that there is no evidence of infection outside of the Fraser Valley in British Columbia.
On 8 December 2014, the Canadian Food Inspection Agency (CFIA) has established a primary control zone in the area where the disease has been identified. The primary control zone is bordered on the west by the Pacific Ocean, on the south by the United States border, on the
north by Highway 16, and on the east by the border between British Columbia and Alberta. Additional information on the zone and the restrictions in place are available at
http://www.inspection.gc.ca/an.imals/te ... 8073267337.
Source of the outbreak(s) or origin of infection
• Unknown or inconclusive
Measures applied
Applied To be applied
• stamping out
• quarantine
• movement control inside the country
• screening
• zoning
• disinfection of infected premises/establishment(s)
Animals treated Vaccination Prohibited
No Yes
Diagnostic test results
Laboratory Type Name of Laboratory Species Test Type Date Results Provided Result
National laboratory National Centre for Foreign
Animal Disease, Canadian Food
Inspection Agency
Birds virus isolation 04/12/2014 Positive
Follow-up report No.: 1, Report reference: CAN-2014-NAI-001., OIE Ref: 16700, Report Date: 09/12/2014, Country: Canada
Printed on: Tue Dec 9 19:41:45 CET 2014 Page 2/4Laboratory Type Name of Laboratory Species Test Type Date Results Provided Result
National laboratory National Centre for Foreign
Animal Disease, Canadian Food
Inspection Agency
Birds intravenous pathogenicity
index (IVPI) test
06/12/2014 Positive
National laboratory National Centre for Foreign
Animal Disease, Canadian Food
Inspection Agency
Birds virus sequencing 04/12/2014 Positive
National laboratory National Centre for Foreign
Animal Disease, Canadian Food
Inspection Agency
Birds real-time reverse
transcriptase/polymerase chain
reaction (RRT-PCR)
03/12/2014 Positive
Future Reporting
The event is continuing. Weekly follow-up reports will be submitted.

http://www.oie.int/wahis_2/public%5C..% ... 194145.pdf

Page 1 of 2 All times are UTC - 5 hours [ DST ]
Powered by phpBB® Forum Software © phpBB Group
http://www.phpbb.com/